DNA Techniques and Analysis Quiz

What is the primary purpose of the heating step in the PCR process?

• To activate the primers

• To denature the DNA strands

• To synthesise new DNA strands

• To cool down the reaction

Which component of PCR is responsible for initiating the synthesis of new DNA strands?

• Free nucleotides

• Primers

• Heat-resistant enzymes

• DNA template

What is the role of free nucleotides in the PCR process?

• To provide energy for the reaction

• To stabilise the DNA strands

• To serve as building blocks for new DNA strands

• To denature the DNA

Which enzyme is commonly used in PCR due to its ability to withstand high temperatures?

• DNA polymerase

• RNA polymerase

• Ligase

• Helicase

What is the main purpose of the cooling step in the PCR process?

• To denature the DNA strands

• To allow primers to anneal to the DNA template

• To synthesise new DNA strands

• To activate the heat-resistant enzyme

What is electrophoresis primarily used for in molecular biology?

• To amplify DNA

• To separate DNA fragments by size

• To sequence DNA

• To synthesise proteins

In electrophoresis, DNA fragments move towards which electrode?

• Positive

• Negative

• Neutral

• None of the above

What is an electropherogram used for?

• To visualise the sequence of DNA

• To amplify DNA

• To synthesise RNA

• To denature proteins

How can DNA profiling be useful in forensic science?

• By identifying genetic disorders

• By determining the sequence of DNA

• By matching DNA samples to individuals

• By amplifying DNA

Which of the following is a potential ethical issue related to the collection of genetic information?

• Increased accuracy in DNA sequencing

• Privacy concerns regarding personal genetic data

• Faster processing of DNA samples

• Improved forensic techniques