Recombinant DNA Technology

What is the first step in the process of recombinant DNA technology?

What role do restriction enzymes play in recombinant DNA technology?

What are some plant applications of recombinant DNA technology?

• Resist disease

• Increase nitrogen fixation

• Contain minerals, vitamins – more nutritious

• Better taste – prevent from rotting before purchase

• Have pesticides – resist insects

Which is the primary purpose of using restriction enzymes before gel electrophoresis?

• It cuts the DNA strands in a set location for analysis.

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

A method used to rapidly make multiple copies of a specific segment of DNA.

• PCR (Polymerse Chain Reaction)

• Electrophoresis

• DNA Probe

What are some animal applications of recombinant DNA technology?

• Leaner meat

• Fish that grow quicker

• More vitamins in food

• More nutritious milk

• Greater milk production

What are some medical applications of recombinant DNA technology?

• Produce human medications or supplements in milk of organisms

• Human insulin produced by E.coli

• Human growth hormone

• Dissolve blood clots

• Human clotting factor

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What are some industry applications of recombinant DNA technology?

• Trees that produce plastics

• Biodegradable plastic wrap, paper products from wheat

• Blue cotton – doesn’t need dyed

• Silk in goat’s milk

• Cotton that doesn’t twist – doesn’t need ironing

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What happens during the ligation of DNA molecules?