Recombinant DNA Technology

What is the first step in the process of recombinant DNA technology?

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What role do restriction enzymes play in recombinant DNA technology?

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What are some plant applications of recombinant DNA technology?

• Resist disease

• Increase nitrogen fixation

• Contain minerals, vitamins – more nutritious

• Better taste – prevent from rotting before purchase

• Have pesticides – resist insects

Which is the primary purpose of using restriction enzymes before gel electrophoresis?

• It cuts the DNA strands in a set location for analysis.

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

A method used to rapidly make multiple copies of a specific segment of DNA.

• PCR (Polymerse Chain Reaction)

• Electrophoresis

• DNA Probe

What are some animal applications of recombinant DNA technology?

• Leaner meat

• Fish that grow quicker

• More vitamins in food

• More nutritious milk

• Greater milk production

What are some medical applications of recombinant DNA technology?

• Produce human medications or supplements in milk of organisms

• Human insulin produced by E.coli

• Human growth hormone

• Dissolve blood clots

• Human clotting factor

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What are some industry applications of recombinant DNA technology?

• Trees that produce plastics

• Biodegradable plastic wrap, paper products from wheat

• Blue cotton – doesn’t need dyed

• Silk in goat’s milk

• Cotton that doesn’t twist – doesn’t need ironing

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What happens during the ligation of DNA molecules?

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