MCQs on Glycolysis

Where does glycolysis occur in the cell?

• Mitochondria

• Cytoplasm

• Nucleus

• Endoplasmic reticulum

What is the first enzyme in the glycolytic pathway?

• Hexokinase

• Phosphofructokinase

• Pyruvate kinase

• Enolase

How many ATP molecules are consumed in the energy preparatory phase of glycolysis?

• 1

• 2

• 4

• 0

What is the net ATP gain from one molecule of glucose in glycolysis?

• 2 ATP

• 4 ATP

• 6 ATP

• 8 ATP

Which of the following enzymes is the rate-limiting enzyme of glycolysis?

• Hexokinase

• Phosphofructokinase-1 (PFK-1)

• Pyruvate kinase

• Aldolase

Which of the following is the end product of glycolysis?

• Acetyl-CoA

• Oxaloacetate

• Pyruvate

• Ribose-5-phosphate

How many NADH molecules are produced per glucose molecule in glycolysis?

• 1

• 2

• 4

• 6

Which of the following enzymes catalyzes a substrate-level phosphorylation reaction?

• Pyruvate kinase

• Phosphoglucoisomerase

• Glyceraldehyde-3-phosphate dehydrogenase

• Phosphofructokinase

Which step in glycolysis is irreversible?

• Glucose → Glucose-6-phosphate

• Fructose-6-phosphate → Fructose-1,6-bisphosphate

• Phosphoenolpyruvate → Pyruvate

• All of the above

Which intermediate in glycolysis is a high-energy compound?

• Glucose-6-phosphate

• 3-Phosphoglycerate

• Phosphoenolpyruvate

• Fructose-6-phosphate