Molecular Biology Quiz

Which of the following is a method for identifying recombinant colonies?

• Gram staining

• Northern blotting

• Blue-white screening

• Western blotting

The lacZ gene in plasmid vectors helps in:

• Protein purification

• Antibiotic resistance

• Recombinant screening

• DNA replication

In blue-white screening, white colonies indicate:

• Non-recombinant plasmids

• Recombinant plasmids

• Dead cells

• Contaminated plates

Which of the following is not a selectable marker?

• Ampicillin resistance

• Tetracycline resistance

• Green Fluorescent Protein (GFP)

• Origin of replication

Colony hybridization involves the use of:

• RNA primers

• Radioactive probes

• Ligases

• Restriction enzymes

Which of the following techniques is used to determine the nucleotide sequence of DNA?

• PCR

• Gel electrophoresis

• Sanger sequencing

• ELISA

In Sanger’s method of DNA sequencing, chain termination is caused by:

• ATP

• dNTPs

• ddNTPs

• DNA ligase

Which enzyme is used in Sanger sequencing to synthesize DNA?

• Taq polymerase

• DNA ligase

• DNA polymerase I

• DNA polymerase

Which of the following is true about a genomic DNA library?

• Contains only coding sequences

• Made from mRNA

• Contains both exons and introns

• Contains only promoter sequences

A cDNA library is constructed using:

• Genomic DNA

• mRNA and reverse transcriptase

• tRNA and DNA ligase

• Plasmids and ligase