hbbb

What is the primary purpose of the Polymerase Chain Reaction (PCR)?

• To visualize DNA

• To amplify DNA

• To extract RNA

• To sequence DNA

Who invented the Polymerase Chain Reaction (PCR)?

• James Watson

• Rosalind Franklin

• Kary Mullis

• Francis Crick

What is the optimum temperature for the extension step in PCR?

• 55-65ºC

• 37ºC

• 72ºC

• 94-96ºC

Which enzyme is primarily used in PCR for DNA synthesis?

• RNA polymerase

• Ligase

• DNAse I

• Taq polymerase

What is the role of primers in PCR?

• To extend DNA strands

• To anneal to DNA strands

• To denature DNA

• To visualize DNA

In agarose gel electrophoresis, what does the size of the DNA fragment affect?

• The temperature of the gel

• The speed of migration

• The color of the DNA

• The type of buffer used

What is the purpose of using a loading dye in agarose gel electrophoresis?

• To track the sample during electrophoresis

• To visualize DNA

• To amplify DNA

• To denature proteins

Which buffer is commonly used in agarose gel electrophoresis?

• Tris-acetate EDTA

• Boric acid

• Sodium chloride

• Phosphate buffer

What is the relationship between agarose gel concentration and pore size?

• Lower concentration leads to larger pores

• Pore size is independent of gel type

• Concentration has no effect on pore size

• Higher concentration leads to larger pores

What is the first step in the DNA extraction process?

• Centrifugation

• Cell lysis

• Precipitation

• Purification