Chromatography Techniques Quiz

Which of the following chromatography techniques separates molecules based on size?

• Ion exchange chromatography

• Gel permeation chromatography

• Affinity chromatography

• Gas chromatography

In ion exchange chromatography, proteins are separated based on their:

• Molecular weight

• Shape

• Net charge

• Solubility

Which chromatography technique uses a specific ligand immobilized on the stationary phase?

• HPLC

• Gel filtration chromatography

• Affinity chromatography

• Gas chromatography

The stationary phase in gas chromatography is typically:

• Gas

• Liquid

• Solid or liquid on a solid support

• Water

Which of the following is NOT typically used in HPLC?

• High pressure pumps

• Flame ionization detector

• UV detector

• Reverse phase column

Hydrophobic interaction chromatography (HIC) separates proteins based on:

• Charge

• Size

• Affinity to a ligand

• Surface hydrophobicity

In gel filtration chromatography, larger molecules elute before smaller ones.

In affinity chromatography, all proteins bind to the stationary phase.

HPLC stands for High Performance Liquid Chromatography.

Gas chromatography is best suited for analyzing large proteins.