Chromatography Techniques Quiz

Which of the following chromatography techniques separates molecules based on size?

• Ion exchange chromatography

• Gel permeation chromatography

• Affinity chromatography

• Gas chromatography

In ion exchange chromatography, proteins are separated based on their:

• Molecular weight

• Shape

• Net charge

• Solubility

Which chromatography technique uses a specific ligand immobilized on the stationary phase?

• HPLC

• Gel filtration chromatography

• Affinity chromatography

• Gas chromatography

The stationary phase in gas chromatography is typically:

• Gas

• Liquid

• Solid or liquid on a solid support

• Water

Which of the following is NOT typically used in HPLC?

• High pressure pumps

• Flame ionization detector

• UV detector

• Reverse phase column

Hydrophobic interaction chromatography (HIC) separates proteins based on:

• Charge

• Size

• Affinity to a ligand

• Surface hydrophobicity

In gel filtration chromatography, larger molecules elute before smaller ones.

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In affinity chromatography, all proteins bind to the stationary phase.

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HPLC stands for High Performance Liquid Chromatography.

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Gas chromatography is best suited for analyzing large proteins.

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