Biochemistry: Metabolism Part 2 Glycolysis

Which phase of glycolysis is referred to as the "investment phase"?

• Pyruvate production

• Steps 1–3, where ATP is consumed

• Steps 4–6, where energy is harvested

• Anaerobic respiration

What enzyme phosphorylates glucose in the first step of glycolysis?

• PFK-1

• Hexokinase

• Enolase

• Aldolase

What is the role of PFK-1 in glycolysis?

• Reversible step in energy harvesting

• Rate-limiting enzyme and major regulatory step

• Forms NADH

• Breaks down pyruvate

What is the net ATP yield from glycolysis per glucose molecule?

• 4

• 0

• 2

• 3

Which metabolite is produced at the end of glycolysis?

• Lactate

• Pyruvate

• Acetyl-CoA

• Glycerol

What process recycles NADH back to NAD+ during anaerobic glycolysis?

• Gluconeogenesis

• Conversion of pyruvate to lactate

• β-oxidation

• TCA cycle

Which enzyme converts DHAP to G3P?

• Aldolase

• GAPDH

• Triose phosphate isomerase

• Pyruvate kinase

Which step in glycolysis directly generates NADH?

• Hexokinase

• Enolase

• Glyceraldehyde-3-phosphate dehydrogenase

• PFK-1

What molecule donates phosphate during substrate-level phosphorylation in glycolysis?

• ATP

• High-energy intermediates like 1,3-BPG and PEP

• GTP

• Water

What is the role of enolase in glycolysis?

• Phosphorylation of glucose

• Dehydration of 2-phosphoglycerate to PEP

• ATP formation

• Oxidation of pyruvate