2025 Unit 8 Quiz 2

CRISPR can edit DNA. What organisms naturally has CRISPR?

• Viruses

• Humans

• Plants

• Bacteria

Why do bacteria have CRISPR? Check all that apply.

• To be able to remember which viruses they have been infected with

• To be able to edit virus DNA

• To cut apart virus DNA thereby stopping the virus infection

• To be able to edit their own DNA

Tags

NGSS.HS-LS4-2

NGSS.HS-LS4-4

What does the cas9 protein do?

• Prevents viruses from entering bacteria

• Cut RNA

• Cut DNA

• Binds to RNA

Where are the "repeats" referred to in CRISPR found, and what are they?

• They are small pieces of DNA found in viruses.

• They are small pieces of viruses found in bacteria's DNA.

• They are small pieces of RNA found in virus DNA.

• They are small pieces of mutations found in DNA.

What are the two results of Cas9 breaking both strands of DNA?

• Rehabilitate a gene or alter a virus

• Disable a gene or fix a mistake

• Repair a gene or make a mistake

• Broken DNA and sad scientists

CRISPR-Cas9 was discovered in which of the following organisms?

• protists

• fungi

• bacteria

• viruses

Write the correct sequence that matches this chromatogram.

What is the purpose of gel electrophoresis in the Sanger sequencing method?

• To amplify DNA fragments

• To cut DNA into smaller fragments

• To separate DNA fragments based on size

• To visualize the DNA directly

How does temperature play a role in the Polymerase Chain Reaction (PCR) used in the Sanger method?

• High temperatures are used to separate the DNA strands

• Low temperatures are used to activate the DNA polymerase

• Constant temperature is maintained throughout the reaction

• Temperature is irrelevant in PCR

Tags

NGSS.HS-PS1-5

In the Sanger sequencing method, what is the purpose of adding a mixture of dNTPs and fluorescently labeled dideoxynucleotides to the reaction? (NIC)

• To ensure that every nucleotide in the DNA sequence is fluorescently labeled

• To terminate DNA synthesis at random positions, creating fragments of varying lengths

• To increase the speed of the DNA polymerase

• To bind the DNA fragments together