Biotech Review

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.

• To copy DNA.

• To create DNA nucleotides.

• To maintain the temperature of the PCR reaction.

Answer explanation

Just as in DNA replication, a primer allows the polymerase a starting point to bind to.

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

Which suspect is likely guilty?

• Suspect #1
• Suspect #2
• Suspect #3

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Based on these results whose blood was found in the blood stain at the crime scene?

• Bob
• Sue
• John
• Lisa

How many restriction sites appear in the picture?

• 1

• 2

• 3

• 4

If the target sequence is GCATTA, what is the complementary sequence?

• CGUAAU

• CGTAAT

• CGTUUT

• GCATTA

Answer explanation

Remember, PCR is a way of doing DNA replication in vitro, or outside of the cell, so there will be no uracil.

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

• convert the DNA into gel

• cut the DNA into fragments

• change the color of the DNA

• produce longer sections of DNA

What is a practical application for the process illustrated?

• Translation and Transcription

• DNA Fingerprinting

• Gene Therapy

• Human Genome Project

What kind of cuts were made in the scenario above?

• Blunt

• Sticky

• No cuts were made

• Palindrome