DNA --> Protein

Transcription is similar to DNA replication in that

• an RNA transcript is synthesized discontinuously and the pieces are then joined together

• it uses the same enzyme as that used to synthesize RNA primers during DNA replication

• the newly synthesized RNA remains paired to the template DNA

• Nucleotide polymerization occurs only in the 5' to 3' direction

RNA polymerase 1

• most rRNA genes

• tRNA genes

• 5S rRNA genes

• protein-coding genes

• miRNA genes

RNA polymerase 2

• most rRNA gnese

• tRNA genes

• 5S rRNA genes

• protein-coding genes

• miRNA genes

RNA polymerase 3

• most rRNA genes

• tRNA genes

• 5S rRNA genes

• protein-coding genes

• miRNA genes

5'-AGTCTAGGCACTGA-3'

3'-TCAGATCCGTGACT-5'

If the polymerase is transcribing from this segment of DNA from left to right, which strand (top or bottom) is the template?

• Top

• Bottom

5'-AGTCTAGGCACTGA-3'

3'-TCAGATCCGTGACT-5'

What will be the sequence of that RNA (be sure to label the 5' and 3' ends of your RNA molecule)?