Soal Pilihan Ganda - PCR dan Elektroforesis

What is the main component in GoTaq® Green Master Mix (2x) that acts as an enzyme for DNA synthesis?

• GelRed

• DreamTaq DNA Polymerase

• TAE buffer

• Primer

What primers are used in this procedure?

• UniB1 and BactF1

• UniF1 and BactR1

• Forward Taq and Reverse Taq

• DNA marker and DNA ladder

What is the initial denaturation temperature in the PCR program?

• 72°C

• 60°C

• 95°C

• 48.2°C

What is the duration of the initial denaturation?

• 10 minutes

• 1 minute

• 3 minutes

• 30 seconds

What is the temperature used during the annealing stage?

• 95°C

• 48.2°C

• 72°C

• 25°C

How many cycles are performed in the PCR program for the 16sRNA gene?

• 10

• 15

• 25

• 35

At what temperature is the final elongation stage performed?

• 50°C

• 95°C

• 72°C

• 65°C

What is one function of Nuclease Free Water in the PCR reaction?

• Increase reaction temperature

• Preserve DNA

• Dissolve reaction components

• Prevent denaturation

What device is used to run the repeated temperature program in PCR?

• Spectrophotometer

• Thermal Cycler

• UV Transilluminator

• Vortex

What is the main function of DNA marker (DNA ladder) in electrophoresis?

• Provide enzymes for PCR reaction

• Stabilize electric voltage

• Determine the size of DNA sample bands

• Absorb UV light