HCC-Review Lab Exam 3: Essay Questions

Carbohydrate fermentation test – Explain.

• A test used to determine the ability of microorganisms to ferment carbohydrates, producing acid and/or gas.

• A test used to measure protein synthesis in bacteria.

• A test to identify the presence of lipids in a sample.

• A test to detect the presence of nucleic acids in microorganisms.

The products of carbohydrate fermentation test are:

• Acids, gases, and sometimes alcohol

• Proteins and lipids

• Vitamins and minerals

• Enzymes and hormones

PH indicator used in Carbohydrate Fermentation test is:

• Phenol red

• Methyl orange

• Crystal violet

• Bromothymol blue

The sugars used in carbohydrate fermentation test are:

• Glucose, lactose, sucrose

• Fructose, maltose, galactose

• Starch, cellulose, glycogen

• Mannose, ribose, xylose

Carbohydrate fermentation – name and identification of Durham tube.

• A small inverted tube used to detect gas production during fermentation.

• A tube used to measure pH changes in fermentation.

• A tube used to store carbohydrate solutions only.

• A tube used for protein precipitation in fermentation.

Purpose of Durham tube in Carbohydrate fermentation is to:

• Detect gas production during fermentation

• Measure pH changes

• Provide nutrients for bacteria

• Indicate color change only

In carbohydrate fermentation test, which two specific criteria were used to differentiate between metabolic capabilities of bacteria?

• Acid production and gas formation

• Colony color and shape

• Temperature tolerance and pH resistance

• Spore formation and motility

TSI stands for:

• Triple Sugar Iron

• Total Serum Iron

• Tissue Specific Index

• Transcriptional Signal Indicator

The purpose of Triple Sugar Iron Test is:

• to differentiate among enteric bacteria based on their ability to ferment sugars and produce hydrogen sulfide

• to measure blood glucose levels in patients

• to detect the presence of viral infections in blood samples

• to determine the antibiotic sensitivity of bacteria

3 sugars name in TSI medium are:

• Glucose, lactose, sucrose

• Glucose, maltose, fructose

• Lactose, maltose, mannitol

• Sucrose, fructose, galactose