Differential Centrifugation and Protein Fractionation Quiz

Differential centrifugation separates cell components based on their:

• Charge

• Solubility

• Size and density

• Shape only

Which organelle pellets first during differential centrifugation of a cell homogenate?

• Mitochondria

• Nucleus

• Ribosomes

• Microsomes

The speed (× g) required to sediment mitochondria is typically:

• 600 × g

• 10,000 × g

• 100,000 × g

• 200,000 × g

Which fraction remains in the supernatant after centrifugation at 100,000 × g?

• Nuclei

• Mitochondria

• Microsomes

• Cytosolic proteins

The term “homogenization” in centrifugation refers to:

• Breaking cells to release organelles

• Dissolving proteins in buffer

• Mixing of organelles with buffer

• Washing organelles

Differential centrifugation is an example of:

• Analytical technique

• Preparative technique

• Chromatographic method

• Electrophoretic method

Which buffer is commonly used in cell homogenization for centrifugation?

• Hydrochloric acid

• Sodium hydroxide

• Phosphate buffer

• Sulphuric acid

At 100,000 × g for 60 minutes, which components sediment?

• Nuclei

• Mitochondria

• Ribosomes and microsomes

• Soluble proteins

Which of the following is NOT required for differential centrifugation?

• Homogenizer

• Refrigerated centrifuge

• Solvent gradient

• Buffer

Which organelle would you isolate at the lowest centrifugal force?

• Nucleus

• Microsomes

• Ribosomes

• Cytosolic proteins