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Hemoglobin and Heme Biosynthesis Quiz

Authored by Kaye Dayoha

Health Sciences

University

Hemoglobin and Heme Biosynthesis Quiz
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20 questions

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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which of the following is the rate-limiting step in heme biosynthesis?

Conversion of porphobilinogen to hydroxymethylbilane

Incorporation of Fe²⁺ into protoporphyrin IX

Formation of δ-aminolevulinic acid from glycine and succinyl-CoA

Conversion of coproporphyrinogen III to protoporphyrinogen IX

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The heme pocket in hemoglobin plays a crucial role by:

Allowing reversible oxidation of Fe²⁺ to Fe³⁺

Protecting Fe²⁺ from oxidation while permitting O₂ binding

Binding 2,3-BPG directly to regulate O₂ affinity

Forming covalent bonds with oxygen molecules

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which hemoglobin variant has the highest oxygen affinity due to weaker interaction with 2,3-BPG?

Hb A

Hb A₂

Hb F

Hb Portland

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

In adult blood, the expected percentage of Hb A₂ is normally up to 3.5%. An Hb A₂ level greater than 3.5% is most indicative of:

α-thalassemia trait

β-thalassemia trait

Iron deficiency anemia

Sickle cell disease

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which statement best describes myoglobin compared to hemoglobin?

It binds O₂ with lower affinity than Hb A

It has a sigmoidal O₂ dissociation curve

It stores O₂ in muscle and has a hyperbolic curve

It transports CO₂ in muscle tissue

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which laboratory method is considered the reference method for hemoglobin measurement?

Oxyhemoglobin method

Hb electrophoresis

Cyanmethemoglobin method

HPLC

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What wavelength is used to measure cyanmethemoglobin in the spectrophotometer?

340 nm

420 nm

540 nm

620 nm

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