Gel Electrophoresis

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

• convert the DNA into gel

• cut the DNA into fragments

• change the color of the DNA

• produce longer sections of DNA

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Which chemicals are used to cut DNA into fragments for a gel electrophoresis procedure?

• enzymes

• molecular bases

• hormones

• ATP molecules

This technique used to analyze DNA involves the

• synthesis of new DNA strands from subunits

• separation of DNA fragments based on size

• production of genetically engineered DNA molecules

• removal of defective genes from DNA

Which suspect is likely guilty?

• Suspect #1
• Suspect #2
• Suspect #3

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

Once DNA has been extracted from the nucleus of a cell, it is exposed to ______ ______ that act like biochemical scissors to cut the DNA.

• restriction enzymes

• gel agarose

• clotting factors

• electric shock