CRISPR-Cas9 Technology and Its Implications

Jennifer Doudna discusses the CRISPR-Cas9 technology, a groundbreaking tool for genome editing that allows precise changes to DNA. Developed with Emmanuelle Charpentier, CRISPR has potential applications in curing genetic diseases and has been tested in various organisms, including human embryos. The technology raises ethical concerns, especially regarding its use in human embryos and potential for genetic enhancement. Doudna calls for a global conversation to address these issues responsibly. The talk highlights the importance of considering both intended and unintended consequences of scientific advancements.

What was the original purpose of the research that led to the development of CRISPR technology?

• To cure genetic diseases

• To understand bacterial immune systems

• To develop new antibiotics

• To create genetically modified organisms

What role does the Cas9 protein play in the CRISPR system?

• It replicates viral DNA

• It cuts and degrades viral DNA

• It transports RNA

• It synthesizes proteins

Why is the CRISPR-Cas9 complex compared to a pair of scissors?

• It can synthesize RNA

• It can replicate DNA

• It can cut DNA at specific sites

• It can bind to proteins

What is a major ethical concern regarding the use of CRISPR technology?

• Its speed of development

• Its potential to create designer humans

• Its complexity

• Its cost

What is one of the first expected applications of CRISPR technology in humans?

• Editing genes in the blood

• Modifying brain cells

• Treating skin diseases

• Enhancing muscle growth

What is a potential benefit of using CRISPR technology in animal models?

• It can create new species

• It can help study human diseases

• It can improve animal intelligence

• It can increase animal lifespan

What is a concern about the commercial interest in CRISPR technology?

• It may lead to monopolies

• It may prioritize enhancement over therapeutics

• It may slow down research

• It may increase costs

What is the purpose of the global pause called by scientists regarding CRISPR technology?

• To improve the technology

• To consider ethical implications

• To increase funding

• To expand its use

What historical precedent is mentioned in relation to the CRISPR pause?

• The discovery of DNA

• The development of antibiotics

• The moratorium on molecular cloning

• The invention of the microscope

What is a potential risk of using CRISPR technology without proper regulation?

• It could cause environmental damage

• It could lead to data breaches

• It could slow down technological progress

• It could result in unintended genetic changes