Overview of Metabolism [Part 4] | Glutamate, Glutamine, & Nucleotide Biosynthesis

The video explores the integration of purine and pyrimidine biosynthesis with basic metabolism. It highlights the regulation of key enzymes involved in these pathways, such as glutamate dehydrogenase and glutamine synthetase. The video explains how purine biosynthesis starts with ribose 5 phosphate and involves several allosteric enzymes, while pyrimidine biosynthesis uses oxaloacetate and ammonia to form carbamoyl aspartate. The tight regulation of these pathways is emphasized, showing their connection to the citric acid cycle and overall metabolic processes.

Which enzyme is responsible for converting alpha-ketoglutarate to glutamate?

• Aspartate transaminase

• Glutamate dehydrogenase

• Carbamoyl phosphate synthetase

• Glutamine synthetase

What is the initial compound required for purine biosynthesis?

• Ribose 5-phosphate

• Oxaloacetate

• Carbamoyl phosphate

• Aspartate

Which enzyme converts ribose 5-phosphate to phosphoryl pyrophosphate in purine biosynthesis?

• Glutamine PRPP aminotransferase

• ATP phosphoribosyl transferase

• Aspartate transcarbamoylase

• Glutaminase

What is the role of aspartate in pyrimidine biosynthesis?

• It is a waste product

• It is converted to glutamine

• It combines with carbamoyl phosphate

• It forms inosine

Which enzyme is allosterically regulated in pyrimidine biosynthesis?

• Aspartate transcarbamoylase

• Glutamine synthetase

• Glutamate dehydrogenase

• ATP phosphoribosyl transferase

What is the precursor to pyrimidines in the biosynthesis pathway?

• Phosphoryl amine

• Carbamoyl aspartate

• Inosine

• Ribose 5-phosphate

How are purine and pyrimidine biosynthesis pathways connected to the citric acid cycle?

• Through the conversion of oxaloacetate to glutamine

• By the formation of ribose 5-phosphate

• Through the production of ammonia

• Via the regulation of allosteric enzymes