Microbiology: Urea Test

Microbiology: Urea Test

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Science, Chemistry, Biology, Health Sciences

University

Hard

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The video tutorial explains the urease test, a biochemical test to determine if bacteria can break down urea into ammonia, which raises the pH and changes the color of phenol red indicator to hot pink. This test is clinically relevant for distinguishing between lactose non-fermenting gram-negative bacilli like Salmonella, Shigella, and Proteus, with only Proteus expressing urease and producing ammonia.

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5 questions

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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the primary purpose of the urease test?

To identify the presence of gram-positive bacteria

To measure the oxygen consumption of bacteria

To check if bacteria can break down urea

To determine if bacteria can ferment lactose

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What does the enzyme urease do in the urease test?

It ferments lactose into lactic acid

It oxidizes glucose into carbon dioxide

It converts ammonia into urea

It breaks down urea into carbon dioxide and ammonia

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How does phenol red indicate a change in pH during the urease test?

It turns green in neutral conditions

It turns yellow in acidic and hot pink in alkaline conditions

It remains colorless in alkaline conditions

It turns blue in acidic conditions

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which bacteria can be distinguished using the urease test?

Lactobacillus, Clostridium, and Bacteroides

Staphylococcus, Streptococcus, and Bacillus

Salmonella, Shigella, and Proteus

Escherichia coli, Salmonella, and Shigella

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What color change indicates the presence of Proteus in the urease test?

Yellow

Hot pink

Green

Blue