Deoxyribose-5-Phosphate Aldolase Physiology, Biochemistry, and Mechanism

The video tutorial explores purine catabolism, focusing on the conversion of deoxyribose 5-phosphate into glyceraldehyde 3-phosphate and acetaldehyde. It details the enzyme mechanisms involved, including nucleophilic attacks and Schiff base formation. The tutorial also covers the energy production from these catabolized products through glycolysis and the TCA cycle, highlighting the generation of NADH and ATP.

What is the initial product formed when deoxyribose 1-phosphate is converted by phospho deoxyribose mutase?

• Phosphoglucomutase

• Deoxyribose 5-phosphate

• Glyceraldehyde 3-phosphate

• Acetaldehyde

Which enzyme mechanism involves a nucleophilic attack by lysine?

• Deoxyribose 5-phosphate aldolase

• Aldolase

• Phosphoglucomutase

• Glyceraldehyde 3-phosphate dehydrogenase

What role does the protonated Schiff base play in the enzyme mechanism?

• Forms a carbon-carbon bond

• Stabilizes a positive charge

• Acts as a nucleophile

• Releases acetaldehyde

During the enzyme mechanism, what is regenerated in the active site?

• Lysine

• Acetaldehyde

• NADH

• Glutamic acid

What is the final product formed from acetaldehyde in the discussed mechanism?

• Acetyl CoA

• Glyceraldehyde 3-phosphate

• ATP

• NADH

Which enzyme is involved in the conversion of glyceraldehyde 3-phosphate during glycolysis?

• Phosphoglucomutase

• Pyruvate kinase

• Glyceraldehyde 3-phosphate dehydrogenase

• Aldolase

What is the energy yield from the catabolism of deoxyribose 1-phosphate?

• Neither ATP nor NADH

• Both ATP and NADH

• Only NADH

• Only ATP