Gluconeogenesis Pathway: Overview, Enzymes, Co-Factors and Precursors

The video tutorial explains gluconeogenesis, the process of synthesizing glucose from non-carbohydrate precursors, crucial during fasting and starvation. It occurs mainly in the liver and kidneys. The tutorial compares gluconeogenesis with glycolysis, highlighting key enzymes and steps, including pyruvate carboxylase and PEP carboxykinase. It discusses the energy requirements, noting that gluconeogenesis consumes six ATP molecules. The regulation of the pathway by hormones like glucagon and the role of precursors such as lactate, amino acids, and glycerol are also covered.

What is gluconeogenesis and why is it important during fasting?

Describe the primary location where gluconeogenesis occurs.

What are the three irreversible steps in glycolysis that gluconeogenesis must bypass?

Explain the role of pyruvate carboxylase in gluconeogenesis.

What is the significance of the malate-aspartate shuttle in gluconeogenesis?

What is the function of fructose bisphosphatase in gluconeogenesis?

Discuss the regulation of gluconeogenesis by glucagon.

Identify the gluconeogenic precursors and their sources.

How does lactate contribute to gluconeogenesis?

How do amino acids contribute to gluconeogenesis?