Citrate Synthase

The video explains the mechanism of citrate synthase, focusing on the conversion of oxaloacetate and acetyl CoA into citrate. It covers the aldol condensation and hydrolysis steps, detailing the role of active site residues like histidine and aspartate. The process involves proton transfer, nucleophilic attack, and the formation of Citroyl-CoA, leading to the final product, citrate.

What happens during the hydrolysis step of the citrate synthase mechanism?

How does citric acid form from the tetrahedral intermediate?

What is the final product of the citrate synthase mechanism?