Acetyl-S-CoA Carboxylase Part 1 (Biotin Carboxylase)

The video tutorial explores the biosynthesis of malonyl CoA, a key molecule in fatty acid biosynthesis. It discusses the role of biotin-dependent carboxylases, the linkage of biotin to lysine, and the importance of bicarbonate in carboxylation. The tutorial also explains the enzyme domains involved in the process and provides a detailed mechanism of carboxylation, highlighting the steps and interactions within the enzyme's active site.

What is the significance of the amid linkage between biotin and lysine in the active site?

What distinguishes the biotin carboxylase domain from the trans carboxylase domain?

How does the rotation of the lysine residue affect the function of the enzyme?

Describe the mechanism by which carbon dioxide is generated in biotin-dependent carboxylases.