Microbiology: MacConkey Agar

The video tutorial explains the use of MacConkey Agar, a selective and differential medium, to identify gram-negative bacteria capable of fermenting lactose into lactic acid. The presence of bile salts and crystal violet inhibits gram-positive bacteria, allowing only gram-negative bacteria to grow. The medium contains a pH indicator that turns hot pink when lactose is fermented into acid end products. The biochemical pathway involves lactose being hydrolyzed into glucose and galactose, which enter glycolysis to form pyruvate, eventually converting to lactic acid. The video also covers interpreting results on MacConkey Agar plates, identifying lactose fermenting and non-fermenting colonies.

What components of Macconkey Agar inhibit the growth of gram-positive bacteria?

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What is the primary purpose of Macconkey Agar?

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What color change indicates the fermentation of lactose in Macconkey Agar?

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Describe the biochemical process that occurs when lactose is fermented into lactic acid.

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Explain how Macconkey Agar differentiates between lactose fermenting and non-lactose fermenting bacteria.

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