Unraveling Recombinant DNA Technology and Its Applications

Recombinant DNA technology involves isolating a DNA fragment from a donor and inserting it into another organism's DNA to introduce new characteristics. The process includes obtaining a DNA fragment, acquiring a plasmid, cutting both with restriction enzymes, and inserting the fragment into the plasmid using DNA ligase. This recombinant plasmid can be used for various purposes, such as producing proteins like human insulin or creating genetically modified organisms.

What is the primary purpose of recombinant DNA technology?

• To create identical copies of an organism

• To introduce new characteristics into an organism

• To remove unwanted genes from an organism

• To study the structure of DNA

What is the first step in recombinant DNA technology?

• Cutting the DNA with restriction enzymes

• Inserting the DNA fragment into a plasmid

• Introducing the recombinant plasmid into a host cell

• Obtaining a DNA fragment with the gene of interest

What role does a plasmid play in recombinant DNA technology?

• It acts as a host cell for the DNA fragment

• It serves as a vector to transfer the gene of interest

• It cuts the DNA at specific points

• It glues the DNA fragments together

Which enzyme is used to cut the DNA fragment and plasmid?

• DNA polymerase

• DNA ligase

• Restriction enzyme

• Helicase

What is the function of DNA ligase in recombinant DNA technology?

• To replicate the DNA fragment

• To cut DNA at specific sequences

• To introduce the plasmid into a host cell

• To join the DNA fragment and plasmid

What can recombinant plasmids be used for?

• Producing proteins like human insulin

• Studying DNA sequences

• Removing unwanted genes

• Creating identical organisms