DNA Extraction Techniques and Processes

This video tutorial explains the process of extracting DNA from plant cells. It covers the preparation of plant tissue by freezing and pulverizing it, followed by cell lysis using a buffer solution and detergent. The video then describes the separation of DNA from other cellular components using chloroform and centrifugation. Finally, it details the precipitation and purification of DNA using ethanol, resulting in pure DNA for molecular techniques.

Why is young leaf tissue considered a good source of DNA?

• Young cells are actively dividing and have fresh DNA.

• It is less expensive to obtain.

• It is easier to handle.

• It contains more chlorophyll.

What is the purpose of freezing the leaf tissue with liquid nitrogen?

• To help mechanically break the cell walls.

• To preserve the color of the leaves.

• To make the tissue easier to cut.

• To enhance the DNA quality.

What tool is used to pulverize the frozen leaf tissue?

• A blender

• A centrifuge

• A knife

• A drill-like tool

What role does detergent play in the DNA extraction process?

• It cools the solution.

• It breaks down the cell membrane.

• It enhances DNA replication.

• It stabilizes the DNA.

At what temperature is the tissue incubated to disrupt the cell?

• 45°C

• 30°C

• 75°C

• 60°C

Why is chloroform added during the DNA extraction process?

• To enhance DNA solubility.

• To increase the temperature.

• To wash away unwanted cell components.

• To color the solution.

What is the purpose of using centrifugal force in the DNA extraction process?

• To freeze the solution.

• To mix the solution thoroughly.

• To separate the solvent layer from the DNA solution.

• To heat the solution.

What happens when cold ethanol is added to the DNA solution?

• The solution becomes warmer.

• The DNA dissolves completely.

• The DNA precipitates out of the solution.

• The solution changes color.

What is the final use of the pure DNA obtained from the extraction process?

• To enhance plant growth.

• To change the color of the plant.

• To study the biological activities of the plant cell.

• To create new plant species.

Which molecular technique can be used with the extracted DNA?

• Transpiration

• Fermentation

• PCR

• Photosynthesis