Plasmid DNA and Gene Therapy Concepts

The video explains the role of cells and proteins in the human body, highlighting how DNA instructions can be altered, leading to genetic diseases. It discusses recent biotech advances that allow for the correction of these mutations using plasmid DNA. Plasmid DNA, naturally found in bacteria, is a key tool in genetic therapy, enabling the correction of defective genes. The video covers in vivo and ex vivo gene therapy techniques, using viruses like AAV and lentivirus to deliver therapeutic DNA. It also explores the use of plasmid DNA in vaccines and the support provided by companies like Aldevron in advancing plasmid-based treatments.

What is the primary role of proteins within cells?

• To regulate bodily functions and structure

• To store genetic information

• To transport oxygen

• To provide energy

How can genetic mutations occur?

• Through exercise

• By consuming certain foods

• Due to hereditary conditions or external factors

• Through regular cell division

What recent advancement allows for the correction of genetic mutations?

• Plasmid DNA technology

• Traditional medicine

• Surgery

• Radiation therapy

What is plasmid DNA?

• A type of virus

• A circular, double-stranded DNA molecule

• A single-stranded RNA molecule

• A type of protein

How do plasmids help bacteria?

• By helping them survive in harsh environments

• By storing nutrients

• By providing energy

• By producing toxins

What is the role of AAV in gene therapy?

• To destroy defective cells

• To deliver therapeutic DNA to infected cells

• To provide nutrients to cells

• To replicate DNA

What is an example of ex vivo gene therapy?

• Antibiotic treatment

• CAR-T cell therapy

• Chemotherapy

• Radiation therapy

What is the purpose of DNA vaccinations?

• To provide nutrients

• To induce immunological responses

• To increase cell division

• To repair damaged tissues

Which company is mentioned as supporting plasmid-based treatment development?

• Moderna

• Aldevron

• Pfizer

• Johnson & Johnson

What is the main benefit of plasmid DNA in scientific research?

• It is versatile and supports various applications

• It is difficult to manipulate

• It is expensive to produce

• It is only used in agriculture