Pipetting Techniques and Accuracy

The video tutorial covers the essential lab skill of pipetting, emphasizing the importance of precision and minimizing contamination. It guides viewers through selecting the appropriate pipette, adjusting it for the correct volume, and installing the correct tip. The tutorial provides detailed instructions on the proper technique for using a pipette, including handling the plunger and liquid. It also explains how to dispense liquid accurately and addresses common issues that can affect accuracy. Finally, it suggests methods for testing pipette accuracy and technique.

Why is it important to know how to use a pipette properly?

• To maximize precision and minimize errors

• To save time in the lab

• To avoid using other lab equipment

• To impress colleagues

What should you consider first when selecting a pipette?

• The color of the pipette

• The volume you need to dispense

• The length of the pipette

• The brand of the pipette

How do you ensure the pipette is set to the correct volume?

• By shaking the pipette

• By using a ruler

• By adjusting the pipette until the correct volume is displayed

• By checking the color code

What is the purpose of pressing down on the plunger before drawing liquid?

• To release air from the pipette

• To calibrate the pipette

• To prevent liquid from entering the pipette

• To ensure the tip is securely attached

Why should the pipette tip remain below the liquid surface when drawing liquid?

• To measure the liquid temperature

• To avoid splashing

• To prevent bubbles from forming

• To keep the pipette clean

At what angle should you hold the container when dispensing liquid?

• 90 degrees

• 60 degrees

• 30 degrees

• 45 degrees

What should you do when you feel resistance while dispensing liquid?

• Shake the pipette

• Pull the pipette out quickly

• Stop immediately

• Keep pressing to ensure all liquid is dispensed

What is a common cause of inaccurate transfer volumes?

• Using a new pipette tip

• Leaky or poorly sealed pipette tips

• Dispensing too quickly

• Using a different liquid

How can you test the accuracy of your pipetting technique?

• By measuring the liquid with a ruler

• By dispensing water onto a scale

• By using a thermometer

• By checking the color of the liquid

What should each 100 microliter drop weigh when testing pipette accuracy?

• 200 milligrams

• 100 milligrams

• 150 milligrams

• 50 milligrams