Complementary DNA Sequences Concepts

This video tutorial explains how to write a complementary DNA sequence from a single DNA strand. It covers the basic rules of DNA strand orientation, base-pairing rules, and the convention of writing nucleotide sequences from the 5-prime to the 3-prime end. The video provides a practical example to help students understand how to correctly write complementary sequences by matching appropriate bases and ensuring the correct orientation.

What is the main focus of this video tutorial?

• How to identify DNA mutations

• How to synthesize DNA strands

• How to extract DNA from cells

• How to write a complementary DNA sequence

What does it mean for DNA strands to be complementary?

• They are identical in structure

• They have the same sequence

• They run in the same direction

• They run in opposite directions

Which base pairs with Adenine (A) in DNA?

• Adenine (A)

• Guanine (G)

• Thymine (T)

• Cytosine (C)

In which direction are nucleotide sequences conventionally written?

• Random order

• From the middle outwards

• 5-prime to 3-prime

• 3-prime to 5-prime

What should you do if a DNA sequence is written from 3-prime to 5-prime?

• Leave it as is

• Ignore the sequence

• Reverse it to 5-prime to 3-prime

• Add more nucleotides

What is the correct orientation for writing a complementary DNA sequence?

• 3-prime to 5-prime

• Any direction

• 5-prime to 3-prime

• From the middle outwards

Why is it unnecessary to indicate the 5-prime and 3-prime ends in a sequence?

• Because they are always implied

• Because they are not important

• Because sequences are always written from 3-prime to 5-prime

• Because sequences are always written from 5-prime to 3-prime

What common mistake do students make when writing complementary sequences?

• Writing from 5-prime to 3-prime

• Writing from 3-prime to 5-prime

• Using incorrect base pairs

• Adding extra nucleotides

What is the final step in writing a complementary DNA sequence?

• Check for mutations

• Ensure the sequence is in 5-prime to 3-prime orientation

• Add a primer

• Synthesize the sequence