Plasmid Mini Prep Procedures

Plasmid Mini Prep Procedures

Assessment

Interactive Video

Biology, Chemistry, Science

10th - 12th Grade

Practice Problem

Hard

Created by

Patricia Brown

FREE Resource

This video tutorial provides a detailed protocol for using the Perial Plasmid Mini Prep System. It covers the processing of bacterial culture, cell lysis, neutralization, centrifugation, and assembly of the mini column. The tutorial also includes steps for endotoxin removal, washing, and the final elution of plasmid DNA, ensuring high DNA yield and purity.

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9 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of the podcast mentioned in the introduction?

To explain the theory behind plasmid DNA

To compare different DNA extraction methods

To discuss the history of plasmid mini prep systems

To provide a detailed protocol for the Perial Plasmid Mini Prep System

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the maximum volume of bacterial culture that can be directly processed using the Perial Plasmid Mini Prep System?

300 microliters

600 microliters

900 microliters

1200 microliters

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is added to the bacterial culture after the cell lysis buffer?

Elution buffer

Neutralization solution

Endotoxin removal wash

Column wash solution

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How long should the centrifugation be performed after adding the neutralization solution?

3 minutes

1 minute

4 minutes

2 minutes

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What should be done with the flow-through after the first centrifugation?

It should be heated

It should be mixed with the next solution

It should be saved for later use

It should be discarded

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of the endotoxin removal wash?

To neutralize the solution

To remove impurities

To increase DNA yield

To elute the DNA

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How much column wash solution is added to the mini column?

200 microliters

400 microliters

100 microliters

300 microliters

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