

pBAD Vector System Concepts
Interactive Video
•
Biology
•
11th - 12th Grade
•
Practice Problem
•
Hard
Patricia Brown
FREE Resource
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10 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the primary function of the pBAD vector system?
To degrade proteins in bacteria
To express recombinant proteins in bacteria
To replicate bacterial DNA
To inhibit bacterial growth
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of maintaining the pBAD vector in a medium lacking arabinose?
To enhance protein expression
To promote bacterial growth
To reduce plasmid instability
To increase plasmid instability
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How is the expression of the gene of interest induced in the pBAD vector system?
By increasing temperature
By adding glucose to the medium
By removing arabinose from the medium
By adding l-arabinose to the medium
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What role does the araBAD promoter play in the pBAD vector system?
It degrades the gene of interest
It drives expression of the gene of interest and AraC
It replicates the plasmid
It inhibits gene expression
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What happens to AraC in the absence of l-arabinose?
It forms a loop blocking transcription
It enhances protein synthesis
It activates transcription
It degrades the promoter
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
How does the addition of glucose affect gene expression in the pBAD vector system?
It increases plasmid stability
It has no effect on gene expression
It enhances gene expression
It suppresses basal expression
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the effect of high cAMP levels in a glucose-free medium?
It reduces plasmid stability
It binds to the bBAD promoter, activating it
It inhibits the bBAD promoter
It degrades the gene of interest
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