Lab 6: Protein Purification Techniques

Lab 6 is a small-scale representation of the final steps in recombinant therapeutic protein production. It begins with creating a liquid bacterial culture, primarily done by the teacher, using various methods depending on the site. Students then lyse the cells to release the RFP protein into the supernatant. Safety and sterile techniques are emphasized, including proper cleaning and waste disposal, which vary by site and must comply with school regulations.

What is the primary focus of Lab 6 in the context of recombinant therapeutic protein production?

• Quality control of protein products

• Middle steps of protein folding

• Final steps of protein purification

• Initial steps of protein synthesis

Who primarily performs the creation of a liquid bacterial culture in Lab 6?

• Students

• Lab technician

• Teacher

• Site coordinator

What is one method mentioned for obtaining a bacterial culture?

• Using a synthetic culture

• Mixing two different colonies

• Plucking a pink colony from a plate

• Using a pre-made culture

What is the first step students perform with their sample in Lab 6?

• Heat the sample

• Measure the pH

• Centrifuge the sample

• Add lysis buffer

Where is the RFP protein located after the first centrifuge step?

• In the supernatant

• In the lysis buffer

• In the cell pellet

• In the air

What color is the cell pellet after the first centrifuge step?

• White

• Green

• Pink

• Blue

After lysing the cells, where is the RFP protein found?

• In the cell debris

• In the buffer solution

• In the supernatant

• In the cell pellet

What should be stressed to students regarding lab safety?

• Wearing colorful lab coats

• Bringing snacks to the lab

• Safety and sterile techniques

• Using the correct pipette

What should be used to clean lab benches before and after experiments?

• Water

• Soap

• Disinfectant

• Vinegar

Who should be contacted for waste disposal protocols?

• ABE site technician or coordinator

• School principal

• Class teacher

• Local government