Microscopy Techniques and Observations

The video tutorial provides a comprehensive guide on using microscopes, including handling, components, and preparing specimens. It covers the steps to prepare an onion specimen, apply iodine stain, and use a coverslip. The tutorial also explains how to focus and view the specimen at different magnifications. Finally, it emphasizes the importance of proper packing and storage of microscopes to prevent damage.

Why is it important to handle microscopes with care?

• They are not used often.

• They are easy to replace.

• They are very heavy.

• They are fragile and expensive.

What is the purpose of the eyepiece lens in a microscope?

• To hold the specimen in place.

• To illuminate the specimen.

• To adjust the focus.

• To magnify the specimen for viewing.

What is the magnification power of the objective lens marked 'x 40'?

• 400 times

• 40 times

• 10 times

• 4 times

Why is iodine used when preparing an onion cell specimen?

• To clean the cells.

• To give the cells color and density.

• To make the cells transparent.

• To make the cells larger.

What is the purpose of a coverslip in microscopy?

• To hold the specimen in place.

• To prevent the lens from touching the liquid.

• To magnify the specimen.

• To protect the specimen from light.

What should you do first when starting to observe a specimen under a microscope?

• Adjust the light source.

• Use the highest magnification.

• Start with the lowest magnification.

• Place the specimen directly on the lens.

What can be observed at 40x magnification of an onion cell?

• Only the cell wall.

• The nucleus and cell membranes.

• The entire onion.

• Only the cell membrane.

What is the final step before removing the slide from the microscope?

• Remove the coverslip.

• Return to the smallest magnification.

• Turn off the light.

• Increase the magnification.

Why is it important to store microscopes properly?

• To make them look neat.

• To prevent them from getting dusty.

• To ensure they are easy to find.

• To avoid breakages.

What should be done with the coverslip after use?

• Reuse it for the next specimen.

• Leave it on the slide.

• Throw it away.

• Clean it and store it.