PEP Carboxykinase

The video tutorial explains the reversal of pyruvate kinase, a highly exergonic reaction in glycolysis, requiring two enzymes: pyruvate carboxylase and phosphoenolpyruvate carboxykinase. The mechanism of phosphoenolpyruvate carboxykinase is detailed, highlighting its GTP dependency and the role of entropy in driving the reaction. The tutorial analyzes the reactants and products, emphasizing the positive delta S and its impact on Gibbs free energy, making the reaction feasible. The video concludes with a recap and notes on the enzyme's dual mitochondrial and cytosolic presence.

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