Graphical Estimation of Kd from P:L Binding Plot

The video tutorial explains the concept of KD, an equilibrium constant related to protein-ligand binding. It introduces the variable Theta, representing the percentage of binding sites occupied by a ligand. The tutorial demonstrates how to estimate KD graphically from binding curves and compares the binding strength of different ligands. It also presents an alternative method to assess binding strength at a specific concentration, emphasizing that a lower KD indicates stronger binding.

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