Microbiology Lab Techniques Quiz

What is the primary purpose of using antibiotic discs in the experiment?

• To observe the inhibitory effects on microorganism growth

• To enhance the growth of microorganisms

• To sterilize the culture

• To change the color of the agar plate

Which tool is used to transfer 100 microliters of culture aseptically?

• Forceps

• Micropipette

• Sterile spreader

• Bunsen burner

Why is it important to flame the neck of the bottle during the aseptic technique?

• To change the color of the culture

• To cool down the culture

• To sterilize the bottle neck

• To heat the culture

What is a cheaper alternative to using a micropipette for spreading culture?

• Using a sterile spreader

• Using a Bunsen burner

• Using Pasteur pipettes and syringes

• Using a microscope

What is the purpose of using filter paper discs in the experiment?

• To test different antimicrobial solutions

• To absorb excess culture

• To increase the temperature of the culture

• To change the color of the agar

Which antimicrobial solution is used as a control in the experiment?

• Veron

• Ellin

• Iodine

• Sterile water

What is the recommended temperature range for incubating the plates?

• 10-15°C

• 20-25°C

• 25-30°C

• 35-40°C

Why is it important to turn the plates upside down during incubation?

• To keep the solution in place

• To ensure even temperature distribution

• To prevent contamination

• To dry the culture faster

What happens if the culture is not dry before applying the discs?

• The culture will not grow

• The solution will run all over the plate

• The agar will melt

• The discs will not stick

How long does it typically take to see results if the plates are incubated at room temperature?

• 24-48 hours

• 12-24 hours

• 72-96 hours

• 48-72 hours