Colony Formation Assay Quiz

What is the primary purpose of the colony formation assay?

• To measure cell size

• To study cell survival and proliferation

• To observe cell movement

• To analyze DNA sequences

Which method can be used to count cells in the preparation of cell cultures?

• Using a spectrophotometer

• Using a centrifuge

• Using a cell-counting chamber

• Using a microscope

What is the purpose of diluting the cell suspension in the preparation step?

• To achieve a specific cell concentration

• To increase cell density

• To enhance cell growth

• To remove dead cells

What is the purpose of leaving two wells untreated during the experiment?

• To test a different drug

• To serve as a control

• To save resources

• To observe contamination

What is the significance of using technical duplicates in the experiment?

• To ensure reproducibility and accuracy

• To reduce the cost of the experiment

• To increase the number of samples

• To test different drugs simultaneously

What is the role of crystal violet in the colony formation assay?

• To fix the cells

• To stain the colonies

• To increase cell proliferation

• To reduce background noise

How are the cells fixed before staining in the colony formation assay?

• With acetone

• With methanol

• With formaldehyde

• With 70% ethanol

Why is it important to set the control to 100% in the data analysis?

• To determine the incubation time

• To compare treated and untreated conditions

• To ensure accuracy in cell counting

• To measure the size of colonies

What can be inferred if mutant cells are more susceptible to drug treatment than wild-type cells?

• Mutant cells are healthier

• Mutant cells have a higher proliferation rate

• Mutant cells may have beneficial mutations for treatment

• Mutant cells are resistant to the drug

What tool is recommended for creating publication-quality graphs from the data?

• GraphPad Prism

• Google Sheets

• Microsoft Excel

• MATLAB