Chromatography Quiz

What is the primary purpose of chromatography?

• To measure the temperature of compounds

• To mix different compounds

• To separate, identify, and purify compounds

• To create new chemical compounds

Which type of chromatography uses Whatman number one filter paper?

• Thin-layer chromatography

• Column chromatography

• Gas chromatography

• Paper chromatography

What is the role of the mobile phase in paper chromatography?

• To carry the analyte along the stationary phase

• To remain stationary

• To dissolve the stationary phase

• To change the color of the analyte

Which solvent mixture is used for optimal separation of plant pigments?

• Ethanol and methanol

• Acetone and petroleum ether

• Water and ethanol

• Methanol and isopropanol

What is the first step in preparing for the chromatography experiment?

• Marking the solvent front

• Cutting the chromatography paper

• Pouring the solvent into the jar

• Grinding the leaves with solvent and sand

How is the chromatography paper prepared before placing it in the jar?

• By drawing a pencil line and applying leaf extract

• By soaking it in water

• By heating it

• By cutting it into small pieces

What should be done once the solvent reaches the desired level on the paper?

• Heat the jar

• Add more leaf extract

• Add more solvent

• Remove the paper and mark the solvent front

Which pigment is not always present in the green leaves used in the experiment?

• Anthocyanin

• Xanthophyll

• Carotene

• Chlorophyll a

How is the retardation factor (Rf) calculated?

• Distance traveled by solvent divided by distance traveled by pigment

• Distance traveled by pigment divided by distance traveled by solvent

• Distance traveled by pigment plus distance traveled by solvent

• Distance traveled by solvent minus distance traveled by pigment

What is the purpose of marking the solvent front on the chromatography paper?

• To identify the type of solvent used

• To measure the distance traveled by the solvent

• To determine the color of the pigments

• To calculate the weight of the paper