Microscopy Techniques Quiz

What is the primary reason for choosing one type of light microscope over another?

• Cost of the microscope

• Availability of the microscope

• Specific research needs

• Ease of use

Which type of microscopy involves fixing and staining cells, resulting in dead specimens?

• Phase contrast microscopy

• Fluorescent microscopy

• Bright field microscopy

• Dark field microscopy

Which microscopy technique allows for the study of cell morphology and external structures?

• Fluorescent microscopy

• Bright field microscopy

• Dark field microscopy

• DIC microscopy

What type of staining uses acidic dyes that are repelled by cells?

• Simple staining

• Differential staining

• Negative staining

• Positive staining

What is a key advantage of dark field microscopy over bright field microscopy?

• It is less expensive

• It requires staining

• It can be used to view living cells

• It provides a 3D image

Which microscopy technique is particularly useful for viewing spirochetes?

• Dark field microscopy

• Bright field microscopy

• DIC microscopy

• Fluorescent microscopy

Which type of microscopy is most suitable for viewing the internal structures of eukaryotic cells without staining?

• Fluorescent microscopy

• Bright field microscopy

• Dark field microscopy

• DIC and phase contrast microscopy

What is the main principle behind fluorescent microscopy?

• Manipulating light wavelengths for 3D images

• Using fluorescent dyes to emit light

• Blocking light to create contrast

• Using heat to fix cells

What is the purpose of using fluorescent antibody assays in microscopy?

• To detect the presence of viruses

• To fix cells to a slide

• To stain cell structures

• To create a 3D image

Which microscopy technique can be used to visualize the cell cytoskeleton?

• Fluorescent microscopy

• DIC microscopy

• Dark field microscopy

• Bright field microscopy