Agarose Gel Electrophoresis Lab Quiz

What is the correct concentration of TAE buffer to use when preparing agarose gel?

• 10x TAE

• 1x TAE

• 5x TAE

• 2x TAE

Why is it important to use masking tape when setting up the casting tray?

• To prevent the gel from solidifying

• To ensure the gel does not leak

• To make the gel more visible

• To increase the gel's conductivity

What is the purpose of adding Red Safe to the gel?

• To prevent the gel from melting

• To increase the gel's strength

• To speed up the gel solidification

• To visualize the DNA

What should you do if bubbles form while pouring the gel?

• Ignore them

• Use a comb to push them to the end

• Add more agarose

• Stir the gel vigorously

Why is it important to handle the gel carefully when removing masking tape?

• The gel will lose its color

• The gel will not solidify otherwise

• The gel will become too hot

• The gel is very fragile and can slide out

What is the role of the loading buffer in DNA sample preparation?

• To help the sample sink to the bottom of the well

• To make the DNA visible

• To increase the DNA's charge

• To prevent the DNA from degrading

What happens if you dispense the DNA sample too soon into the well?

• The sample will evaporate

• The sample will become contaminated

• The sample will float

• You may lose some of the sample

Why is it important to ensure the DNA runs towards the red electrode?

• DNA is positively charged

• DNA is negatively charged

• The red electrode is more stable

• The red electrode is cooler

What indicates that the electrophoresis setup is working correctly?

• The gel changes color

• Bubbles form in the buffer

• The gel becomes opaque

• The power pack turns off

What is the purpose of using a light box in gel visualization?

• To change the gel's color

• To dry the gel

• To illuminate the DNA bands

• To heat the gel