Agarose Gel Electrophoresis Concepts

Agarose Gel Electrophoresis Concepts

Assessment

Interactive Video

Biology, Chemistry, Science

9th - 10th Grade

Hard

Created by

Patricia Brown

FREE Resource

This video tutorial covers the process of agarose gel electrophoresis, a technique used to separate DNA, RNA, proteins, and other biomolecules. It explains the preparation of agarose gel and TAE buffer, the safe handling of ethidium bromide, and the setup of the electrophoresis apparatus. The video also demonstrates how to load DNA samples, run the gel, and visualize the results using a gel documentation system.

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10 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the primary purpose of agarose gel electrophoresis?

To degrade proteins

To amplify RNA

To separate biomolecules based on size

To synthesize DNA

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the first step in preparing agarose gel?

Pouring the gel into the tray

Weighing the agarose

Adding ethylene bromide

Heating the solution

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Why is ethylene bromide added to the agarose gel?

To make DNA visible under UV light

To solidify the gel

To increase the gel's density

To prevent contamination

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the role of the comb in the gel tray?

To cool the gel

To mix the solution

To measure the gel's thickness

To create wells for loading samples

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What safety precaution is necessary when handling ethylene bromide?

Wearing gloves

Using a face shield

Using a fume hood

Wearing a lab coat

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of adding glycerol to the loading dye?

To increase the sample's density

To enhance the sample's fluorescence

To change the sample's color

To decrease the sample's viscosity

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What voltage is typically used to run the gel electrophoresis?

150 volts

100 volts

50 volts

200 volts

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