Pre Lab: Leaf Disk Assay Procedure

Water and Carbon Dioxide

Sugars and Oxygen

Light and Heat

Proteins and Lipids

The absorption of water by the leaf cells.

The production of oxygen gas during photosynthesis.

The consumption of carbon dioxide by the leaves.

The increase in temperature of the water.

To make the water more acidic.

To provide a source of carbon dioxide for photosynthesis.

To help the leaf disks sink initially.

To prevent bacterial growth in the water.

To provide nutrients to the leaves.

To make the leaf surfaces less hydrophobic, allowing water to infiltrate.

To increase the rate of carbon dioxide production.

To act as a disinfectant for the leaves.

Small veins

Leaf edges

Big veins

The middle of the leaf

Distilled water and salt solution

Sodium bicarbonate solution and soapy water

Glucose solution and plain water

Acidic solution and basic solution

To sterilize the leaf disks.

To remove air from the leaf's internal air spaces.

To increase the temperature of the solution.

To prevent the solution from evaporating.

The leaf disks change color.

The leaf disks sink to the bottom of the cup.

The leaf disks float to the surface of the solution.

The solution becomes cloudy.