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Phases of the Cell Cycle - Printable Science Worksheets 9th - 10th Grade - Wayground

Phases of the Cell Cycle

Ivana Grant

Created by Ivana Grant

17 questions

9th - 10th Grade

Science

Updated May 2026

Phases of the Cell Cycle is a free printable Biology worksheet for Grades 9–10 that uses 17 multiple-choice questions to build students’ understanding of how cells grow, copy DNA, divide, and regulate division. Students distinguish interphase, S phase, mitosis, and cytokinesis; identify pictured stages of mitosis; and recognize the role of uncontrolled cell division in cancer. The worksheet also asks students to sequence the stages of mitosis and the broader cell cycle, then apply their knowledge to cell-cycle checkpoints, including the number of checkpoints and the first G1 checkpoint. These varied questions help students connect phase names with observable cell-division events and the order in which those events occur. A complete answer key is included for efficient review, independent practice, or assessment.

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Phases of the Cell Cycle

Total questions: 17

Name
Class
Date
1.
During which phase of the cell cycle does the cell spend the most time?
During which phase of the cell cycle does the cell spend the most time?
a)
Mitosis
b)
Cytokinesis
c)
Interphase
2.
During which part of the cell cycle does the cell divide?
During which part of the cell cycle does the cell divide?
a)
Interphase
b)
Mitosis
c)
S phase
3.
During which part of the cell cycle is DNA copied?
During which part of the cell cycle is DNA copied?
a)
Mitosis
b)
Cytokinesis
c)
S phase
4.
During which parts of the cell cycle does the cell grow?
During which parts of the cell cycle does the cell grow?
a)
G1 and G2
b)
G1 and S
c)
M phase and cytokinesis
5.
Which phase of mitosis is shown in the picture?
Which phase of mitosis is shown in the picture?
a)
Prophase
b)
Anaphase
c)
Telophase
6.
Which phase of mitosis is shown in the picture?
Which phase of mitosis is shown in the picture?
a)
Interphase
b)
Metaphase
c)
Telophase
7.
Which phase of mitosis is shown in the picture?
Which phase of mitosis is shown in the picture?
a)
Prophase
b)
Metaphase
c)
Anaphase
8.
Which phase of mitosis is shown in the picture?
Which phase of mitosis is shown in the picture?
a)
Prophase
b)
Telophase
c)
Metaphase
9.
What is shown in the picture?
What is shown in the picture?
a)
Mitosis
b)
Interphase
c)
Cytokinesis
10.

Uncontrolled cell division which usually leads to tumors and disease is called:

a)

Metaphase

b)

Cytokinesis

c)

Cancer

d)

Diabetes

11.
Put the following stages of mitosis in order: anaphase, prophase, metaphase, and telophase. 
a)
prophase, metaphase, telophase, anaphase
b)
prophase, anaphase, metaphase, telophase
c)
prophase, metaphase, anaphase, telophase
d)
prophase, telophase, metaphase, anaphase
12.
Put the following stages of the cell cycle in order: G2, S, G1, M. 
a)
G1, S, G2, M
b)
S, G1, G2, M
c)
G1, M, G2, S
d)
M, G1, S, G2
13.

What is the purpose the the cell cycle checkpoints?

a)

To repair damaged cells before they enter cell division.

b)

To remove damaged cells from an organism.

14.

How many cell cycle checkpoints are there?

a)

1

b)

2

c)

3

d)

4

15.

What is the first checkpoint of the cell cycle?

a)

Mitosis checkpoint

b)

G1 checkpoint

c)

G2 checkpoint

16.
This is the cell cycle checkpoint that makes sure DNA has been replicated correctly during S phase.
a)
G1 Checkpoint
b)
G2 Checkpoint
c)
M Checkpoint
d)
I Checkpoint
17.
This is the cell cycle checkpoint that makes sure the correct number of chromosomes are present and attached to microtubules.
a)
G1 Checkpoint
b)
G2 Checkpoint
c)
M Checkpoint
d)
I Checkpoint
WaygroundAnswer Key

Phases of the Cell Cycle

Total questions: 17

1.
c) Interphase
2.
b) Mitosis
3.
c) S phase
4.
a) G1 and G2
5.
a) Prophase
6.
b) Metaphase
7.
c) Anaphase
8.
b) Telophase
9.
c) Cytokinesis
10.
c)

Cancer

11.
c) prophase, metaphase, anaphase, telophase
12.
a) G1, S, G2, M
13.
a)

To repair damaged cells before they enter cell division.

14.
c)

3

15.
b)

G1 checkpoint

16.
b) G2 Checkpoint
17.
c) M Checkpoint

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FAQs

What does the Phases of the Cell Cycle worksheet cover?

This Grade 9–10 Biology worksheet uses 17 multiple-choice questions to assess cell-cycle phases, DNA copying in S phase, cell growth in G1 and G2, mitosis, cytokinesis, and cell-cycle checkpoints. Students identify mitosis stages from pictures, sequence both mitosis and the full cell cycle, and connect uncontrolled cell division with cancer.

How can I use this worksheet to teach the phases of the cell cycle?

Introduce the cycle by having students distinguish interphase from M phase, then review what occurs during G1, S, and G2 before discussing mitosis and cytokinesis. As students complete the picture-based mitosis questions, ask them to identify the visual event that marks prophase, metaphase, anaphase, or telophase. Finish by using the sequencing and checkpoint questions to reinforce the cycle as an ordered process rather than a list of vocabulary terms.

What mistakes should I watch for when students complete this cell-cycle worksheet?

Students may confuse interphase with a resting period even though the worksheet treats it as the longest part of the cycle, or select mitosis instead of S phase for DNA copying. In the picture questions, watch for confusion between metaphase, when chromosomes are aligned, and anaphase, when they separate; students may also place telophase before anaphase when ordering mitosis. Checkpoint items may reveal confusion about the three checkpoints and the G1 checkpoint’s position.

How do I assign and grade this Phases of the Cell Cycle worksheet?

The worksheet is available as a printable PDF and as a digital quiz on Wayground, and it includes a complete answer key for the 17 multiple-choice questions. You can grade printable submissions by scanning student work with the Wayground for Teachers app, or use the digital quiz for online response collection and scoring.

How can I differentiate this cell-cycle worksheet for my students?

In the worksheet’s Advanced Settings, adjust font spacing and font size to make the multiple-choice questions and mitosis image prompts easier to read. You can also enable a dyslexia-friendly font or translate the worksheet into another language while preserving its phase-identification, sequencing, and checkpoint practice.

Where can I find more worksheets like this on Wayground?

Wayground offers a comprehensive collection of free printable worksheets and practice problems across subjects, with downloadable PDFs and answer keys to support essential skills and concepts. Browse more resources at https://wayground.com/en-us/worksheets.

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