NEW
Font size
S
M
L
XL
WorksheetsLab quiz wrap-up
Total questions: 55
Worksheet time: 28mins
Name
Class
Date
1.
Which of the following are considered buffering agents and used in buffered formalin
a)
Oxalic acid and sodium oxalate
b)
Formic acid and potassium citrate
c)
Disodium hydrogen phosphate and sodium dihydrogen phosphate
d)
Sodium monobasic phosphate and sodium citrate
2.
What is a characteristic of a good fixative?
a)
Makes tissues more permeable to subsequent reagents.
b)
Preserves tissue in a manner that is not life-like
c)
Enhances putrefaction
d)
Does not harden tissues quickly
3.
Where does chemical fixation begin?
a)
Center and procedures outward
b)
Center and proceeds inward
c)
Periphery and proceeds outward
d)
Periphery and proceeds inward
4.
What is temperature of the tissue processor and embedding center set at to ensure that paraffin wax remains molten?
a)
54-58 degrees C
b)
2 to 4 degrees C above the melting point of the wax
c)
2 to 4 degrees C below the melting point of the wax
d)
62 degrees C
5.
What are the glands that are most associated with hair follicles?
a)
Eccrine sweat glands
b)
Apocrine sweat glands
c)
Sebaceous glands
d)
Sebum glands
6.
Formalin pigment is generally formed in tissues fixed in formalin when the pH:
a)
Rises above 6
b)
Falls below 6
c)
Is neutral
d)
7.2
7.
Why is glacial acetic acid useful in compound fixatives?
a)
It precipitates the cytoplasm
b)
It slows the hardening process.
c)
it counteracts the shrinkage effects of other reagents
d)
it raises the pH of the solution
8.
Improper preservation of tissue can be caused by:
a)
a delay in fixation
b)
rapid penetration of the fixing fluid
c)
prolonged storage in 70% ethanol following fixation
d)
an 8 hour tissue processing schedule
9.
Why is dehydration necessary when processing tissue?
a)
clearing agents do not remove water
b)
paraffin does not infiltrate aqueous tissue
c)
the water in the tissue must be removed
d)
the clearing agent must be removed prior to wax infiltration
10.
Why is dehydration necessary when processing tissue?
a)
clearing agents do not remove water
b)
paraffin does not infiltrate aqueous tissue
c)
the water in the tissue must be removed
d)
the clearing agent must be removed prior to wax infiltration
11.
What is a key property of a clearing agent?
a)
miscible with water and paraffin wax
b)
non-flammable
c)
miscible with alcohol and paraffin wax
d)
non-toxic
12.
How should a Fallopian tube be embedded?
a)
flat
b)
on end
c)
on edge
d)
parallel
13.
For which of the following is the use of vacuum infiltration beneficial?
a)
placenta
b)
skin
c)
appendix
d)
gastric biopsy
14.
What is a characteristic of a tissue processor?
a)
does not require monitoring of temperatures
b)
should be programmed monthly
c)
requires a cleaning cycle after each processing schedule
d)
should be placed in a well-ventilated area of the main histology lab
15.
Prolonged dehydration in higher grades of alcohol will cause the tissue to be what?
a)
hard
b)
porous
c)
toxic
d)
soft
16.
what happens to the tissue when clearing takes place?
a)
alcohol is removed and replaced with xylene
b)
complete dehydrtion is ensured
c)
tissues become translucent
d)
all of the above
17.
What could happen if you take too long to embed a cassette/block?
a)
the tissue sticks too much to the bottom of the mold
b)
The tissue forms cracks
c)
bubbles appear in the tissue block
d)
striations appear in the tissue block
18.
what type of microtome is used for routine sectioning?
a)
sledge
b)
rotary
c)
rocking
d)
cryostat
19.
What is the thickness that most sections are cut at?
a)
1-2 microns
b)
3-4 microns
c)
4-5 microns
d)
3-4 mm
20.
In relation to the melting point of the wax, the temperature of the drying oven should be set at:
a)
5° C below melting point
b)
60 degrees C
c)
same as the melting point of wax
d)
70 degrees C
21.
What temperature should waterbaths be kept at?
a)
8 - 10 degrees C below the melting point of wax
b)
2-3 degrees C above the melting point of wax
c)
2-3 degrees C below the melting point of wax
d)
37 degrees C
22.
Where is the main lock located on the microtome?
a)
on the coarse trimming wheel
b)
attached to the knife blade holder
c)
just below the fine trimming wheel
d)
at the back of the microtome
23.
What is the purpose of floating a tissue ribbon on a bath of 20% ethanol prior to floating it on a water bath?
a)
to see if the section is complete
b)
to remove folds
c)
to provide additional dehydration
d)
to soften the ribbon
24.
The speed of sectioning paraffin blocks on the microtome should be:
a)
fast
b)
slow
c)
variable but even
d)
it doesn't matter
25.
what is the purpose of adding gelatin to the waterbath?
a)
It is antibacterial and prevents bacterial growth
b)
it helps the tissue sections to adhere better to the slide
c)
it prevents carryover
d)
it prevents folds
26.
What are microtome blades made of?
a)
lead
b)
steel
c)
aluminum
d)
tungsten
27.
What could be the cause of this happening? Look carefully.
a)
Temperature of the water bath is too high
b)
Temperature of the water bath is too low
c)
Ribbon has been floating on the water bath too long.
d)
There was a bubble in the block
28.
This is a small biopsy section. what is the name of the artifact?
a)
score
b)
chatter
c)
thick and thin
d)
folds
29.
What happened during course trimming?
a)
bubbles
b)
chatter
c)
scoring
d)
moth eaten
30.
Here sections from two different cases are being floated-out simultaneously. Why would that be a problem?
a)
there is a bubble on one ribbon
b)
cases can get mixed up
c)
contamination could occur
d)
no problem and quicker to do
31.
what is the problem with these two paraffin blocks?
a)
Bubble in the block
b)
striations dure to slow technique
c)
tissue has been incompletely dehydrated and is sunken
d)
autolysis has set in the tissue
32.
The projections of dermis upwards, indenting the bottom of the epidermis, are called:
a)
arrector pili
b)
epidermal appendages
c)
dermal papillae
d)
sweat ducts
33.
Epithelial tissue has a high rate of _________ in order to repair itself quickly.
a)
Epithelial tissue has a high rate of _________ in order to repair itself quickly.
b)
Blood vessels
c)
mitosis
d)
nerve synapses
34.
Looking at the mucosa what part of the digestive system is this picture taken from?
a)
esophagus
b)
stomach
c)
small bowel
d)
large bowel
35.
What intestinal layers is responsible for peristalsis?
a)
muscosa
b)
muscularis mucosa
c)
muscularis propria
d)
submucosa
36.
What is indicated by the arrow?
a)
mucosa
b)
submucosa
c)
muscularis mucosa
d)
crypts of Lieberkuhn
37.
The following is true about the tongue EXCEPT:
a)
has papillae
b)
is covered by simple columnar epithelium
c)
has skeletal muscle
d)
has taste buds
38.
The muscosa of the digestive tract contains a lining epithelium, muscularis mucosa and a layer of connective tissue called:
a)
basal lamina
b)
lamina propria
c)
stratum germavatum
d)
subcutin
39.
What artifact may be seen in stained tissue sections if a prolonged delay in fixation occurred?
a)
the nuclear staining would be stronger
b)
there are white cloudy spots present over the tissue
c)
there are brown black deposits on top of the bloody areas of the tissue
d)
the presence of bacteria may be seen
40.
What does the term "artifact" mean in histotechnology?
a)
The presence of a natural phenomenon.
b)
a structure that was not normally present in living tissue
c)
something that was intentionally done to the tissue to preserve it
d)
added to the slide to enhance staining
41.
what is this histological structure?
a)
adipose tissue
b)
alveoli
c)
bronchiole
d)
clara cell
42.
Identify this structure?
a)
alveolus
b)
respiratory bronchous
c)
terminal bronchiole
d)
bronchus
43.
Which part of the conductive portion of the respiratory system resembles the trachea in its histological organization?
a)
respiratory epithelium
b)
alveoli
c)
bronchus
d)
bronchiole
44.
Which of these structures has no cartilage around it?
a)
tertiary bronchus
b)
terminal bronchiole
c)
primary bronchus
d)
trachea
45.
what histological area is the larger pale cuboidal cells?
a)
proximal tubules
b)
distal tubules
c)
conducting ducts
d)
thick loop of henle
46.
What is the fibrous connective tissue that surrounds each kidney?
a)
bowman's capsule
b)
medulla
c)
cortex
d)
renal capsule
47.
What is the squiggly pink line in the picture?
a)
tunica elastica
b)
internal elastic lamina
c)
endothelium
d)
tunica intima
48.
What is the epithelium of mesothelium and endothelium?
a)
simple cuboidal
b)
simple columnar
c)
simple squamous
d)
serosa
49.
identify the histological area indicated by the arrow?
a)
t. adventitia
b)
t. media
c)
muscular artery
d)
epicarium
50.
Identify this organ:
a)
spleen
b)
tonsil
c)
lymph node
d)
appendix
51.
What organ is this?
a)
parathyroid
b)
thyroid
c)
adrenal
d)
salivary gland
52.
What is this structure in the spleen?
a)
red pulp
b)
white pulp
c)
trabeculae
d)
reticular nodule
53.
What is the adrenal medulla secrete?
a)
aldosterone
b)
norepinephrine
c)
cortisol
d)
androgens
54.
What is the squiggly pink line in the picture?
a)
principal cell
b)
oxyphil
c)
parafollicular cells
d)
follicular cell
55.
What is this structure in the thymus?
a)
amylacea corpora
b)
hassal's corpuscles
c)
oxyphil
d)
c cell
Reset
