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DNA Testing in Forensics

Total questions: 37

Worksheet time: 26mins

Name
Class
Date
1.
What is the complementary strand for GGAGTC
a)
GGAGTC
b)
CCACTG
c)
CCTCAG
d)
GGTGAC
2.
What adds nucleotides to build the complementary strand of DNA
a)
heat
b)
primers
c)
ladders
d)
DNA polymerase
3.
This causes the DNA to "unzip" during PCR
a)
enzymes
b)
primers
c)
heat
d)
ladders
4.
What causes DNA strands to move during gel electrophoresis
a)
electricity
b)
heat
c)
enzymes
d)
primers
5.
The TOTAL number of chromosomes found in humans
a)
23
b)
46
c)
92
d)
64
6.
A version of a gene would be called
a)
codon
b)
primer
c)
standard
d)
allele
7.
This type of DNA testing examines the number of repeats that occur at a particular locus
a)
karyotype
b)
PCR
c)
RLFP
d)
STR
8.

This type of DNA testing involves cutting the DNA into fragments that can later be separated by length

a)

karyotype

b)

PCR

c)

RLFP

9.
This technique is used to separate DNA or protein fragments based on their length
a)
Gel electrophoresis
b)
PCR
c)
RLFP
d)
STR
10.

This technique is used to copy target segments of DNA

a)

Gel electrophoresis

b)

PCR

c)

RLFP

d)

STR

11.
Which of the following proceedures would I do first when testing DNA
a)
Gel electrophoresis
b)
Extract the DNA
c)
PCR
12.
Which of the following proceedures would I do last when testing DNA
a)
Gel electrophoresis
b)
Extract the DNA
c)
PCR
13.
Which is the first step of DNA extraction?
a)
isolate concentrated DNA
b)
burst open cells
c)
collect DNA sample
d)
separate DNA from proteins
14.
Which is the last step in DNA extraction?
a)
isolate concentrated DNA
b)
burst open cells
c)
collect DNA sample
d)
separate DNA from proteins
15.
What causes DNA strands to move during gel electrophoresis?
a)
heat
b)
enzymes
c)
electricity
d)
probes
16.
DNA has what charge?
a)
positive
b)
negative
c)
neutral
17.
What cuts the DNA in RFLP testing?
a)
probes
b)
restriction enzymes
c)
DNA polymerase
d)
heat
18.
What is the database of DNA profiles?
a)
AIFIS
b)
IBIS
c)
ILRC
d)
CODIS
19.

How many loci are included in the FBI database of DNA profiles?

a)

13

b)

8

c)

20

d)

5

20.
DNA should never be packaged in...
a)
paper
b)
plastic
21.
Which of the following DNA "matches" is strongest?
a)
1 in 3 million
b)
1 in 3 billion
c)
1 in 3,000
d)
1 in 300,000
22.
Homozygous means
a)
2 different alleles
b)
2 copies of the same allele
23.
Heterozygous means
a)
2 different alleles
b)
2 copies of the same allele
24.
A known DNA sample I can use to match my unknown sample to a individual is called
a)
probe
b)
primer
c)
isolate
d)
exemplar
25.
A sample of DNA of known lengths run in the gel that can be used as a ruler to measure your DNA fragments
a)
probes
b)
primers
c)
ladders
d)
enzymes
26.

Who matches the crime scene DNA?

a)

suspect #1

b)

suspect #2

c)

suspect #3

d)

none of the suspects match the DNA

27.

Who can be included as source of paternal DNA for this child?

a)

male #1

b)

male #2

c)

neither male

28.

What is the function of DNA polymerase?

a)

to "unzip" the double stranded DNA

b)

to attach to the DNA and act as a primer

c)

an enzyme that adds complementary nucleotides to the unzipped DNA strand

29.

What is the purpose of DNA extraction?

a)

to make copies of the DNA so there is enough to test

b)

to separate the DNA segments by size

c)

to isolate the DNA from the cell

d)

to pull out repeating regions of DNA

30.

What is the purpose of gel electrophoresis?

a)

to make copies of the DNA so there is enough to test

b)

to separate the DNA segments by size

c)

to isolate the DNA from the cell

d)

to pull out repeating regions of DNA

31.

What is the purpose of PCR?

a)

to make copies of the DNA so there is enough to test

b)

to separate the DNA segments by size

c)

to isolate the DNA from the cell

d)

to pull out repeating regions of DNA

32.

Which fragments would move the farthest in gel electrophoresis?

a)

a 12 base pairs long fragment

b)

a 54 base pairs long fragment

c)

a 108 base pairs long fragment

33.

This image shows a

a)

electropherogram

b)

gel electrophoresis

c)

PCR

d)

electrocardiogram

34.

At which locus is this individual homozygous? (check any that apply)

a)

D3S1358

b)

vWA

c)

D16S539

d)

CSF-1PO

e)

TPOX

35.

At which locus is this individual heterozygous? (check all that apply)

a)

D3S1358

b)

vWA

c)

D16S539

d)

CSF-1PO

e)

TPOX

36.

To calculate the probability that an individual is homozygous for 24 repeats at the FGA locus which formula would you use?

a)

P = A2

b)

P = 2AB

37.

To calculate the probability that an individual is heterozygous for 22 and 25 repeats at the FGA locus which formula would you use?

a)

P = A2

b)

P = 2AB

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