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Biotechnology

Total questions: 20

Worksheet time: 23mins

Name
Class
Date
1.

Which technique would most likely be used by forensic scientists?

a)

gene cloning

b)

gene therapy

c)

DNA fingerprinting

d)

karyotyping

2.

Which is one reason scientists produce transgenic organisms?

a)

to create new species of organisms

b)

to control microorganisms in the soil

c)

to prevent habitats from being destroyed

d)

to treat certain types of diseases

3.

Two people believe they are related. Which would be the best technique to determine if they are related?

a)

testing blood types

b)

comparing DNA

c)

examining karyotypes

d)

testing for genetic disorders

4.

Which is a step in the process of producing transgenic bacteria?

a)

A plasmid is used to replace a faulty gene in a human cell.

b)

A chain of bacterial amino acids is inserted into human DNA.

c)

A human gene is inserted into a bacterial plasmid.

d)

A mutation is produced in a bacterial cell.

5.

The process of removing DNA from cells is called

a)

DNA fingerprinting

b)

DNA extraction

c)

Recombinant DNA

d)

Transgenics

6.

Which process is used to make many copies of a DNA sample?

a)

gel electrophoresis

b)

DNA sequencing

c)

Restriction enzyme producing DNA fragments

d)

PCR

7.

What is the function of gel electrophoresis?

a)

to separate DNA fragments according to size and charge

b)

to cut DNA

c)

to recombine DNA

d)

to extract DNA

8.

During transformation,

a)

a prokaryote is changed into a eukaryote.

b)

a cell takes in DNA from outside the cell.

c)

plasmids are inserted into a human cell.

d)

a cell is mutated.

9.

A small circular molecule of DNA found in bacteria cells is called a

a)

chromosome

b)

recombinant DNA

c)

genetic marker

d)

plasmid

10.

DNA produced by combining DNA from two different organisms is known as

a)

a mutant.

b)

a hybrid.

c)

a polyploidy.

d)

Recombinant DNA.

11.

Organisms that contain genes from other organisms are called

a)

transgenic

b)

donor organisms

c)

mutagenic

d)

cloned organisms

12.

Which of the following is an example of uses for bacterial transformation technique?

a)

to produce pure human insulin for use by people with diabetes

b)

to identify a suspect based on evidence from a crime scene

c)

to determine the nucleotide sequence of desired gene

d)

to identify a genetic disorder in preparation for potential treatment

13.

The Human Genome Project is an attempt to

a)

make a DNA fingerprint of every person's DNA.

b)

identify, sequence and catalog all human DNA.

c)

create human-like creatures.

d)

identify alleles in human DNA that are recessive.

14.

The purpose gene therapy in treating disorders , such as cystic fibrosis and SCIDS, is to

a)

replace faulty genes with normal ones

b)

determine the sequence of genes

c)

remove mutations from genes

d)

change dominant alleles to recessive alleles

15.

Which of the following is a concern about the use of genetically modified organisms (GMOs) as food?

a)

they may taste better than normal food

b)

they may cause death if eaten

c)

they can cause allergies, toxins, new diseases and nutritional problems

d)

they are perfectly healthy

16.

What is the function of a restriction enzyme?

a)

to keep DNA from replicating

b)

to cut DNA into smaller fragments

c)

to restrict enzymes from working

d)

to speed up the reaction of DNA polymerase

17.

The restriction enzyme, ECORI, cuts DNA between which two nitrogenous bases?

a)

between guanine (G) and cytosine (C)

b)

between adenine (A) and thymine (T)

c)

between guanine (G) and adenine (A)

d)

between thymine (T) and cytosine (C)

18.

What are DNA fingerprints used for?

a)

to identify the patterns in a person's fingerprint

b)

to show evolutionary relationships

c)

to sequence the DNA bases

d)

crime scene investigations and to determine paternity/maternity

19.

Which DNA technology would best help endangered species?

a)

sequencing

b)

cloning

c)

electrophoresis

d)

antibody production

20.

What type of cells contain plasmids?

a)

eukaryotes

b)

animal cells

c)

prokaryotes

d)

plant cells