WorksheetsIRP Quiz (H)
Total questions: 35
Worksheet time: 18mins
Rod-shaped cells are called:
cocci
bacilli
spirochetes
viruses
Spherical bacteria are called:
cocci
bacilli
spirochetes
viruses
Which one of the following statements about gram-positive and gram-negative bacteria is true?
Gram-positive are spirochetes and gram-negative bacteria are cocci
Gram-negative bacteria have a thicker peptidoglycan layer than gram-positive bacteria
Gram-positive bacteria have a plasma membrane and gram-negative bacteria do not
Gram-negative bacteria have an outer membrane and gram-positive bacteria do not
Gram-positive bacteria:
turn purple when stained with crystal violet
turn pink when stained with crystal violet
are not able to absorb crystal violet
are only able to absorb crystal violet if it's deionized
What is LB agar?
A nutritionally enriched medium made of seaweed
A gelatinous substance that can be used to grow bacteria
A food source for bacteria
All of the above
How would you classify the bacteria shown here?
Gram-positive
Gram-negative
Inconclusive
Spirochete
What form of reproduction do bacteria use?
Asexual reproduction (mitosis)
Asexual reproduction (meiosis)
Sexual reproduction (mitosis)
Sexual reproduction (meiosis)
What can be seen on the agar plate below?
Individual bacterial cells
Gram-positive bacteria
Bacterial colonies
Egg cells being fertilized
About how long does it take for bacteria to replicate?
20 hrs
20 min
20 sec
20 days
Which one of the following processes must come FIRST for gram-staining?
Cover slide with crystal violet for 1 min
Suspend bacterial colony in water on microscope slide
Rinse slide with ethanol
Look at bacteria under microscope
Which step of DNA sequencing comes comes first?
Growing bacteria in a liquid culture (liquid agar)
PCR (Polymerase Chain Reaction)
Gel Electrophoresis
Sending out samples to GeneWiz to be sequenced
What is the goal of making a liquid culture of bacteria?
To replicate the 16S ribosomal subunit so we can have enough samples to send out and sequence
To make sure we have replicated the correct gene
To send it directly to Genewiz to be sequenced
To begin to break apart the bacterial colonies into cells and begin to multiply
What is the goal of PCR?
To make sure we have replicated the correct gene
To amplify (make millions of copies of) a specific gene
To transfer bacterial colonies from the petri dishes to a liquid medium so that they can break apart into cells & begin to multiply
To break down the cells into DNA so that they can be replicated
What is the goal of gel electrophoresis?
To make sure the correct gene sequence has been copied
To amplify (make millions of copies of) a specific gene
To transfer bacterial colonies from a petri dish to a liquid medium so they can break apart into cells & begin to multiply
To break down the cells into DNA so they can be replicated
What does PCR stand for?
Polynucleotide Chain Reaction
Polysaccharide Carbohydrate Redundancy
Polymerase Culture Reaction
Polymerase Chain Reaction
What is the purpose of placing the liquid culture Eppendorf tubes into a thermomixer?
To replicate millions of copies of the bacterial DNA
To break down the colonies into cells so they may begin to multiply
To mix several types of bacterial DNA together into one solution
To make sure that the 16S Ribosomal subunit has been copied correctly
What is the function of a thermocycler?
It aerates the liquid cultures in order to break apart bacterial cells into colonies, so they may begin to multiply
It's used during PCR to replicate the bacterial cells
It's used during PCR to replicate a specific gene sequence into millions of copies
It uses electrodes and a gel in order to separate DNA fragments
What are the correct components of the "Master Mix" that we used throughout PCR in order to replicate the 16S ribosomal subunit?
DNA polymerase, Nitrogen bases, Sterilized water, & Primers
DNA polymerase, Primers, Iodine, Starch, & Sterilized water
RNA polymerase, Nitrogen bases, Sterilized water, & Primers
Primers, Loading dye, Ladder, & Sterilized water
How much of the liquid culture did we add to the PCR tubes that were pre-filled with master mix?
20ul
2ul
48ul
10ul
What is the correct order of events (of replication) that occur during PCR?
Denaturation, Extension, Annealing
Annealing, Extension, Denaturation
Thermocycling, Denaturation, Extension
Denaturation, Annealing, Extension
What occurs during the "denaturation" stage of PCR?
Primers attach to the DNA template
DNA is unwound/unzipped
Nucleotides are added onto the growing DNA strand
Bacterial cells are broken down so the DNA can be accessed
What occurs during the "Annealing" stage of PCR?
Primers attach to the DNA template
Nucleotides are added onto the growing DNA strande
DNA is unwound/unzipped
Bacterial cells are broken down so the DNA can be accessed
What occurs during the "Extension" stage of PCR?
Primers attach to the DNA template
Nucleotides are added onto the growing DNA strand
Bacterial cells are broken down so the DNA can be accessed
DNA is unwound/unzipped
What 2 liquids were combined and placed into the wells of the gels during gel electrophoresis?
Ladder + Loading dye
Sterilized water + Loading dye
Ladder + Sterilized water
PCR product + Loading dye
Which one of the following statements about gel electrophoresis is TRUE?
Positively charged DNA moves towards the negative electrode of a gel
The larger DNA fragments travel farther through the gel than the smaller DNA fragments
Negatively charged DNA moves towards the positive electrode of a gel
As the DNA travels through the gel, it's able to multiply and make millions of copies
Approximately how large is the 16S ribosomal subunit gene?
300 base pairs
750 base pairs
900 base pairs
1500 base pairs
Which company is going to be sequencing our DNA for us?
Cambridge Analytica
GeneWiz
MIT Analytics
Complete Genomics
Which one of the following components does NOT need to be included in your final lab reports?
Abstract
Results
Sources of Error
Sequencing
Which one of the following statements about your IRP lab report is FALSE?
All raw data should be included in the results section
Each data table & graph should have a detailed title
Each data table & graph should have a descriptive paragraph written underneath it
Your hypothesis should be included within the introduction
Which option below is an example of quantitative data?
57% increase in bacterial colony growth
Small, white, translucent bacterial colonies
Gram-positive bacteria
Which one of the following options includes information that should NOT be in your discussion section of your IRP lab writeup?
Whether or not your hypothesis was supported or refuted
Sources of error
Explanations with supporting background research
Graphs and Data tables
What is the correct option for listing the name of a specific bacteria?
Streptococcus epidermidis
Streptococcus Epidermidis
Streptococcus epidermidis
Streptococcus Epidermidis
What is the correct protocol for discarding used petri dishes?
Fill them with sterilized water and discard in the trash
Cover the agar/bacteria with a 10% bleach solution & allow to sit for 20 minutes before discarding in trash
Cover petri dishes, discard in a trash can, and spray with bleach
Scrape agar/bacteria out of petri dish with a gloved hand and discard in trash
At what temperature were your petri dishes incubated at, in order to ensure bacterial replication?
72 F
12 C
32 F
37 C
Which one of the following statements is TRUE?
All petri dishes must be stored in the incubator agar side up, but in the fridge they must be stored agar side down
Petri dishes must be stored agar side up at all times
As long as there is no condensation inside the petri dishes, they can be stored agar side down
The covers of the petri dishes must be removed before placing them into the incubator
