WorksheetsMicro lab #1
Total questions: 36
Worksheet time: 18mins
Is this bacteria capsule positive or capsule negative?
Capsule positive
Capsule negative
Is this bacteria capsule positive or capsule negative?
Capsule positive
Capsule negative
Is this bacteria endospore positive or endospore negative?
Endospore positive
Endospore negative
An endospore negative bacterial smear LACKS...
pink vegetative cells
green spores
Is this organism gram-positive or gram-negative?
Gram-positive
Gram-negative
Is this organism gram-positive or gram-negative?
Gram-positive
Gram-negative
Which of the following is TRUE of endospores?
They are metabolically inactive
Produced by only certain groups of bacteria
Formed under conditions of stress (eg. nutrient deprivation, high cell density)
All of the above
What stain do we use to stain endospores?
Crystal violet
Safranin
Malachite green
Copper sulfate
Gram's iodine is a...
Primary stain
Mordant
Decolorizing agent
Counterstain
Where on the microscope do we rest our glass specimen slide?
Illuminator (Light source)
Field diaphragm
Stage
Condenser
At the 40x and 100x objectives, which knob(s) should we use?
Fine adjustment knob
Course adjustment knob
Both
What is the total magnification of a compound light microscope of ocular 10x and objective 40x?
10x
40x
400x
1000x
Which of the following is an accurate description of these colonies?
Red, umbonate, entire margin convex circular colonies
Yellow, entire margin convex pinpoint colonies
Glossy, irregular margin flat colonies
Filamentous and filiform margin
What is the colony elevation of these colonies?
Convex
Raised
Flat
Umbonate
What is the broth growth pattern here?
Precipitation and Pellicle
Turbid
Flocculent
No bacterial growth
What is the broth growth pattern here?
Turbid
Pellicle
Flocculation
Precipitation
Which is best for long-term storage of bacteria?
lyophilization (freeze-drying) or Deep freezing w/glycerol (-70 to - 80C)
Refrigeration
Agar plates
Agar slants
Which of the following is an example of a disinfectant?
Bleach (Clorox)
Penicillin
Hand sanitizer
Iodine
What type of fungal hyphae are present?
Septate
Non-septate
What is this yeast cell doing?
Mitosis
Meiosis
Karyogamy
Budding
What fungal Phylum does this organism belong to?
Basidiomycota
Ascomycota
Zygomycota
What fungal Phylum does this organism belong to?
Basidiomycota
Ascomycota
Zygomycota
What do we call the sac like structures of this organism?
conidiophore
basidia
asci
zygospore
What do we call these structures carrying basidiospores on the gills of this fungal cap?
aerial hyphae
stipe
sporangiophore
basidia
These yeast cells were stained with DAPI, that binds to DNA. Are they eukaryotic or prokaryotic?
Eukaryotes
Prokaryotes
These bacterial rods were stained with DAPI, that binds to DNA. Are they eukaryotic or prokaryotic?
Eukaryotes
Prokaryotes
Can you identify the bacteria vs. buccal cells in this picture?
Bacteria are smaller cocci and rods
Buccal cells have nuclei
The nuclei stains darker b/c DNA is negatively charged
All of the above
What are these yeast cells doing?
Dividing
Budding
Shmooing
What is TRUE of the Plate Count Method?
You can only count organisms that you can grow
It takes time b/c plates have to incubate
Sensitive to low concentrations of bacteria
All of the above
Which disinfectant is MOST effective against P. aeruginosa?
10% bleach
70% Ethanol
3% hydrogen peroxide
All are equally effective
The area of killed bacterial growth around an antibiotic or disinfectant disc is called...
killed zone
dead zone
zone of inhibition
bacterial lawn
Which of the following stains is NOT heat-fixed?
Gram stain
Endospore stain
Capsule stain
Which of the following stains uses multiple dyes to distinguish between multiple features of an organism?
Simple stain
Differential stain
What is the purpose of incubating our bacteria in skim milk broth for the Capsule Stain?
Milk proteins provide contrast
Milk broth promotes capsule growth
Both
Which has a HEPA (high efficiency particulate air) filter?
Biosafety cabinet
Fume hood
Which of the following exhausts air outside the building?
Fume hood
Biosafety cabinet
