WorksheetsBIOTECHNOLOGY: PRINCIPAL AND PROCESSES
Total questions: 20
Worksheet time: 10mins
MOLECULAR SCISSORS USED IN GENETIC ENGINEERING IS-
DNA LIGASE
DNA POLYMERASE
HELICASE
RESTRICTION ENDONUCLEASE
Construction of a recombinant DNA involves
Cleaving and rejoining DNA SEGMENT with ligase alone
Cleaving DNA segment with ligase and joining them with endonuclease
Cleaving DNA segment with endonuclease and joining them with ligase
cleaving and rejoining DNA segment with endonuclease alone
Which is used extensively for genetic engineering in plants?
Agrobacterium tumefaciens
Xanthomonas citri
Bacillus coagulens
Clostridium septicum
Restriction endonucleases cut
single stranded DNA
single stranded RNA
Double stranded DNA
Double stranded RNA
The enzymes which are commonly used in genetic engineering are
Restriction endonuclease and polymerase
Endonuclease and ligase
Restriction endonuclease and ligase
Ligase and polymerase
Identify the plasmid
EcoRI
pBR322
AIUI
Hind II
Ti- Plasmid is obtained from
Azotobacter
Agrobacterium
Yeast
Rhizobium
PCR is required for
DNA synthesis
DNA amplification
Protein synthesis
Amino Acid synthesis
DNA fragments are joined by
Ligase
polymerase
Helicase
Gyrase
Who discovered recombinant DNA technology
Hargobind Khorana
James D. Whatson
S. Cohen and H. Boyer
Sutton and Avery
Which sequence can act as a restriction site
AACCGG
TTGGCC
GGTTGG
CCAACC
AAGGCT
TTCCGA
CTGCAG
GACGTC
GAATTC IS RECOGNITION site for restriction endonuclease
Hind III
EcoR I
Bam I
Hae III
Satellite DNA is useful tool in
sex determination
forensic science
genetic engineering
organ transplantation
Agarose extracted from sea weeds finds use in
PCR
Gel electrophoresis
Spectrophotometry
Tissue culture
Which one of the following techniques made it possible to genetically engineer living organisms
Heavier isotope labelling
Hybridization
Recombination DNA techniques
X-ray diffraction
The process of separation and purification of expressed protein before marketing is called
Upstream processing
Downstream processing
Bio-processing
Post -production processing
Biolistic is suitable for
Transformation of plant cell
DNA fingerprinting
Disarming pathogen vectors
Constructing recombinant DNA by joining with vectors
In pBR 322vector of E.coli which option correctly identifies its components
ori- original restriction enzyme
rop-Reduced osmotic pressure
Hind III, EcoR i - selectable markers
ampR, tetR - Antibiotic resistance genes
Restriction endonuclease
Break DNA at specific sites
Create sticky ends
Produce cross-overs
Both A and B
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Herbert Boyer
Hargovind Khorana
Kary Mullis
Arthur Kornberg
