NEW
Font size
WorksheetsDecalcification
Total questions: 10
Worksheet time: 5mins
This bone forms the shafts of long bones and the bones of the skull
Cancellous bone
Spongy bone
Cortical bone
Trabecular bone
Staining techniques required are often considered when:
Choosing an appropriate decalcifier
Determining the end point of decalcification
The age of a patient is not known
Decalcifying cortical bone
Overdecalcification results in:
Loss of fixative
Reduced cytoplasmic staining
Dried out tissue
Decreased nuclear basophilia
Prior to placing specimens in the decalcifier, the tissue must undergo:
Water rinse
Fixation
Rapid stain
Paraffin embedding
To mechanically determine the decalcification end point, the grosser would:
Arrange for radiography
Complete the calcium oxalate test
Pierce the specimen
Time the decalcification process
An example of cancellous bone is:
Femur
Bone marrow
Rib
Small bones of the fingers
The ideal decalcifier for the bones of this skeleton is:
Nitric Acid
Hydrochloric Acid
Formic acid
Picric Acid
Using heat to decalcify tissue will result in:
Ideal preservation of nuclei
Rapid loss of calcium salts resulting in maceration of other tissue components
Accelerated decalcification with minimal damage
Embedding difficulties
Chemical methods are the most accurate for determining the endpoint of decalcification.
True
False
Enzymes are best preserved with:
Formic Acid
High pH decalcifiers
Nitric Acid
EDTA
