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WorksheetsMolecular Biology Techniques Test 2
Total questions: 20
Worksheet time: 10mins
What are the necessary inducer to start the transcription in prokaryotes?
Sigma factor and holoenzyme
Haloenzyme
Sigma factor and core enzyme
RNA polymerase
Arrange the steps for transcription in decreasing order.
initiation - elongation - termination
initiation - termination - elongation
termination - initiation -elongation
termination - elongation - initiation
All the statement are TRUE about Rho factor EXCEPT
binds to the transcription terminator pause site
function by wrapping nucleic acid around the single cleft
essential transcription protein in prokaryotes
unwinding the RNA transcript from DNA template in elongation step
What is lac repressor?
glucose sensor
start acting as repressor when glucose is present
block transcription of operon in certain condition
bind to isomer of allalactose
What is catabolite activator protein?
lactose sensor
sense glucose through hunger signal, cAMP
active the transcription when the glucose is high
stop the transcription of the operon when the glucose is low
How to start or initiate the lac operon function?
Glucose is available, lactose not available
Sucrose not available, pentose is available
Pentose not available, sucrose is available
Glucose not available, lactose is available
These are the region of lac operon EXCEPT
cAMP site
operator
promoter
lacZ, lacY and lacA
When the low level of transcription occur?
Glucose present, lactose absent
Glucose absent, lactose absent
Glucose present, lactose present
Glucose absent, lactose present
When the strong transcription occur?
Glucose present, lactose absent
Glucose present, lactose present
Glucose absent, lactose absent
Glucose absent, lactose present
What is the condition that activate CAP?
cAMP levels are high because glucose is absent
cAMP levels are low because glucose is absent
cAMP levels are low because glucose is present
cAMP levels are high because glucose is present
What is the definition of transcription?
Synthesis of cDNA molecule with a base complementary to DNA
Synthesis of DNA molecule with a base complementary to DNA
Synthesis of RNA molecule with a base complementary to DNA
Synthesis of protein molecular with a base complementary to RNA
These are the function of RNA polymerase EXCEPT
bind to promoter region on the DNA
unwind the DNA double helix
start chain from stretch
stop the transcription
RNA transcript grow in which direction
5' - 3'
3' end
3' - 5'
5' end
These are the stop codon sequence EXCEPT
UAA
UAG
AUG
UGA
What is the amino acid code for start codon?
Phe
Met
Leu
Ile
Name of the enzyme that cut out the intron
Splicosome
Rho independent
RNA polymerase
Rho dependent
Why we need exon only in translation?
exon is coding sequence
to start initiation
to start the transcription
to terminate the transcription
What is anticodon?
base duplet on tRNA that carry one amino acid
not complementary pair with codon on mRNA
base triplet on mRNA that carry one amino acid
base triplet on tRNA that carry one amino acid
What are the amino acid produced from these two codons, CGU and CGA?
Arg and Ala
Ser and Pro
Phe and Leu
Ala and Asp
Name the interaction between amino acid in protein synthesis
Peptide bond
Hydrogen bond
Covalent bond
van Der Waals
